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Literature summary for 3.2.1.14 extracted from

  • Ikeda, M.; Miyauchi, K.; Mochizuki, A.; Matsumiya, M.
    Purification and characterization of chitinase from the stomach of silver croaker Pennahia argentatus (2009), Protein Expr. Purif., 65, 214-222.
    View publication on PubMed
Search for more literature for 3.2.1.14

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.521
-
 glycol chitin pH 2.5-4.0, 37°C Pennahia argentata

Metals/Ions

Metals/Ions Comment Organism Structure
NaCl 1.5 to 3.0fold activation, dependent on the substrate overview Pennahia argentata

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
 x * 42000, SDS-PAGE Pennahia argentata

Organism

Organism UniProt Comment Textmining
Pennahia argentata
-
 silver croaker fish
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 49fold by ammonium sulfate fractionation, anion exchange and adsorption chromatography, and hydrophobic interaction chromatography Pennahia argentata

Source Tissue

Source Tissue Comment Organism Textmining
stomach
-
 Pennahia argentata
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Pennahia argentata
3.33
-
 purified enzyme, substrate 4-nitrophenyl N-acetylchitobioside Pennahia argentata

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl N-acetylchitobioside + H2O
-
 Pennahia argentata 4-nitrophenol + N-acetylchitobiose
-
 ?
4-nitrophenyl N-acetylchitohexaoside + H2O
-
 Pennahia argentata ?
-
 ?
4-nitrophenyl N-acetylchitopentaoside + H2O
-
 Pennahia argentata ?
-
 ?
4-nitrophenyl N-acetylchitotetraoside + H2O
-
 Pennahia argentata ?
-
 ?
4-nitrophenyl N-acetylchitotrioside + H2O
-
 Pennahia argentata ?
-
 ?
colloidal chitin + H2O
-
 Pennahia argentata ?
-
 ?
glycol chitin + H2O
-
 Pennahia argentata ?
-
 ?
additional information the chitinase shows a wide substrate specificity for degrading alpha-chitin of shrimp and crab shell and beta-chitin of squid pen, substrate specificity silver croaker chitinase preferentially hydrolyzes the 2nd glycosidic link from the non-reducing end of N-acetylglucosaminyl oligomers, no activity with 4-nitrophenyl-GlcNAc, overview Pennahia argentata ?
-
 ?

Subunits

Subunits Comment Organism
? x * 42000, SDS-PAGE Pennahia argentata

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
 assay at Pennahia argentata

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
10 80 temperature profile, overview Pennahia argentata

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
10 40 10 min, purified enzyme, completely stable Pennahia argentata
70
-
 10 min, purified enzyme, loss of 50% activity Pennahia argentata
80
-
 10 min, purified enzyme, loss of over 90% activity Pennahia argentata

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.33
-
 glycol chitin pH 2.5-4.0, 37°C Pennahia argentata

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5
-
 with substrate 4-nitrophenyl N-acetylchitobioside Pennahia argentata
4
-
 with substrate colloidal chitin Pennahia argentata

pH Range

pH Minimum pH Maximum Comment Organism
2.5 8 maximal activity at pH 2.5 and pH 4.0, dependent on the substrate, 20% of maximal activity pH 8.0, inactivation at pH 2.0, overview Pennahia argentata

pI Value

Organism Comment pI Value Maximum pI Value
Pennahia argentata isoelectric focusing
-
 6.2