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Literature summary for 3.2.1.14 extracted from
- Characterization of cold-responsive extracellular chitinase in bromegrass cell cultures and its relationship to antifreeze activity (2008), Plant Physiol., 147, 391-401.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | CHT1 expression is upregulated by exposure to 35°C, but not by salt or osmotic stress, abscisic acid, or ethephon. BiCHT1 mRNA does not accumulate in response to methyl jasmonate and salicylic acid, but is slightly increased by prolonged culture at 25°C and only transiently by chitin | Bromus inermis |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene BiCHT1, functional overexpression in Escherichia coli, functional and expression in transgenic bromegrass cells using Agrobacterium-mediated transformation, recombinant enzymes do not show anti-freeze activity in the expression systems, overview | Bromus inermis |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | the chitinase secreted into the culture medium | Bromus inermis | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 32000 | - |
x * 32000, SDS-PAGE | Bromus inermis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Bromus inermis | the native enzyme is involved in cold acclimation and freezing tolerance, recombinant enzymes do not show anti-freeze activity in the expression systems, overview | ? | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bromus inermis | B1B6T0 | cv. Manchar, cold-responsive chitinase gene BiCHT1 | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell suspension culture | nonembryogenic | Bromus inermis | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | the native enzyme is involved in cold acclimation and freezing tolerance, recombinant enzymes do not show anti-freeze activity in the expression systems, overview | Bromus inermis | ? | - |
? | |
| soluble ethylene glycol chitin + H2O | - |
Bromus inermis | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 32000, SDS-PAGE | Bromus inermis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| antifreeze protein | - |
Bromus inermis |
| CHT1 | - |
Bromus inermis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Bromus inermis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Bromus inermis |
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Release 2023.1 (January 2023)
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