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Literature summary for 3.2.1.14 extracted from

  • Han, Y.; Yang, B.; Zhang, F.; Miao, X.; Li, Z.
    Characterization of antifungal chitinase from marine Streptomyces sp. DA11 associated with South China Sea sponge Craniella australiensis (2009), Mar. Biotechnol., 11, 132-140.
    View publication on PubMed
Search for more literature for 3.2.1.14

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis Streptomyces sp. DA11

Inhibitors

Inhibitors Comment Organism Structure
Ba2+
-
 Streptomyces sp. DA11
EDTA
-
 Streptomyces sp. DA11
EGTA
-
 Streptomyces sp. DA11
Fe2+
-
 Streptomyces sp. DA11
SDS
-
 Streptomyces sp. DA11
theophylline
-
 Streptomyces sp. DA11
Urea
-
 Streptomyces sp. DA11

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.019
-
 colloidal chitin pH 8.0, 50°C Streptomyces sp. DA11
0.078
-
 powdered chitin pH 8.0, 50°C Streptomyces sp. DA11

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ activates Streptomyces sp. DA11
Mg2+ activates Streptomyces sp. DA11
Mn2+ activates Streptomyces sp. DA11
additional information optimal salinity for chitinase activity is 45 g psu Streptomyces sp. DA11

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34000
-
 x * 34000, SDS-PAGE Streptomyces sp. DA11

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Streptomyces sp. DA11 the chitinase C shows antifungal activities against Aspergillus niger and Candida albicans. Streptomyces sp. strain DA11 is associated with South China sponge Craniella australiensis and might be involved in chitin degradation and antifungal defense of the sponge via its chitinase activity ?
-
 ?

Organism

Organism UniProt Comment Textmining
Streptomyces sp. DA11 B0LS89 fragment; marine strain
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 6.15fold by ammonium sulfate fractionation, chitin affinity and anion exchange chromatography Streptomyces sp. DA11

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.95
-
 purified enzyme Streptomyces sp. DA11

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
colloidal chitin + H2O preferred substrate Streptomyces sp. DA11 ?
-
 ?
additional information the chitinase C shows antifungal activities against Aspergillus niger and Candida albicans. Streptomyces sp. strain DA11 is associated with South China sponge Craniella australiensis and might be involved in chitin degradation and antifungal defense of the sponge via its chitinase activity Streptomyces sp. DA11 ?
-
 ?
powdered chitin + H2O
-
 Streptomyces sp. DA11 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 34000, SDS-PAGE Streptomyces sp. DA11

Synonyms

Synonyms Comment Organism
ChiC
-
 Streptomyces sp. DA11
chitinase C
-
 Streptomyces sp. DA11

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Streptomyces sp. DA11

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Streptomyces sp. DA11

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 9 stable, purified enzyme Streptomyces sp. DA11