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Literature summary for 3.2.1.14 extracted from

  • Zakariassen, H.; Aam, B.B.; Horn, S.J.; Varum, K.M.; Soerlie, M.; Eijsink, V.G.
    Aromatic residues in the catalytic center of chitinase A from Serratia marcescens affect processivity, enzyme activity, and biomass converting efficiency (2009), J. Biol. Chem., 284, 10610-10617.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.14

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type and mutant enzymes in Escherichia coli Serratia marcescens

Protein Variants

Protein Variants Comment Organism
F396A site-directed mutagenesis of the +2 subsite residue of isozyme ChiA, the mutant shows only modest reductions in processivity, and shows reduced efficiency toward chitin and an up to 20fold higher activity toward chitosan Serratia marcescens
W167A site-directed mutagenesis of the -3 subsite residue of isozyme ChiA, the mutant shows almost abolished progressivity, reduced efficiency toward chitin and an up to 20fold higher activity toward chitosan Serratia marcescens
W167A/W275A site-directed mutagenesis of the -3 and +1 subsites residues of isozyme ChiA, the mutant shows reduced efficiency toward chitin and an up to 20fold higher activity toward chitosan Serratia marcescens
W275A site-directed mutagenesis of the +1 subsite residue of isozyme ChiA, the mutant shows only modest reductions in processivity, and shows reduced efficiency toward chitin and an up to 20fold higher activity toward chitosan Serratia marcescens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
chitin + H2O Serratia marcescens
-
 ?
-
 ?
chitin + H2O Serratia marcescens BJL200
-
 ?
-
 ?
chitosan + H2O Serratia marcescens
-
 ?
-
 ?
chitosan + H2O Serratia marcescens BJL200
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Serratia marcescens
-
 isozyme ChiA
-
Serratia marcescens BJL200
-
 isozyme ChiA
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Serratia marcescens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chitin + H2O
-
 Serratia marcescens ?
-
 ?
chitin + H2O crystalline beta-chitin, molecular weight analysis of oligomer products, overview Serratia marcescens ?
-
 ?
chitin + H2O
-
 Serratia marcescens BJL200 ?
-
 ?
chitin + H2O crystalline beta-chitin, molecular weight analysis of oligomer products, overview Serratia marcescens BJL200 ?
-
 ?
chitosan + H2O
-
 Serratia marcescens ?
-
 ?
chitosan + H2O soluble chitin-derivative chitosan, molecular weight analysis of oligomer products, overview Serratia marcescens ?
-
 ?
chitosan + H2O
-
 Serratia marcescens BJL200 ?
-
 ?
chitosan + H2O soluble chitin-derivative chitosan, molecular weight analysis of oligomer products, overview Serratia marcescens BJL200 ?
-
 ?
additional information aromatic residues in the catalytic center of chitinase A affect processivity, enzyme activity, and biomass converting efficiency. Progressivity of isozymes is dependent on the substrate, the processive mechanism is essential for an efficient conversion of crystalline substrates but comes at a large cost in terms of intrinsic enzyme speed, overview Serratia marcescens ?
-
 ?
additional information aromatic residues in the catalytic center of chitinase A affect processivity, enzyme activity, and biomass converting efficiency. Progressivity of isozymes is dependent on the substrate, the processive mechanism is essential for an efficient conversion of crystalline substrates but comes at a large cost in terms of intrinsic enzyme speed, overview Serratia marcescens BJL200 ?
-
 ?

Synonyms

Synonyms Comment Organism
ChiA
-
 Serratia marcescens
chitinases A
-
 Serratia marcescens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Serratia marcescens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3
-
 assay at Serratia marcescens