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Literature summary for 3.2.1.14 extracted from

  • Shirota, K.; Sato, T.; Sekiguchi, J.; Miyauchi, K.; Mochizuki, A.; Matsumiya, M.
    Purification and characterization of chitinase isozymes from a red algae, Chondrus verrucosus (2008), Biosci. Biotechnol. Biochem., 72, 3091-3099.
    View publication on PubMed

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
24500
-
x * 24500, isozymes ChiA and ChiC, SDS-PAGE, x * 25500, isozyme ChiB, SDS-PAGE Chondrus verrucosus
25500
-
x * 24500, isozymes ChiA and ChiC, SDS-PAGE, x * 25500, isozyme ChiB, SDS-PAGE Chondrus verrucosus

Organism

Organism UniProt Comment Textmining
Chondrus verrucosus
-
isozymes ChiA, ChiB, and ChiC
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme by ammonium sulfate fractionation, dialysis, anion exchange and hydroxyapatite chromatography, followed by another step of anion exchange chromatography Chondrus verrucosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CM-chitin-RBV + H2O polymeric substrate CM-chitin-RBV Chondrus verrucosus ?
-
?
GlcNAcbeta(1-4)GlcNAc + H2O
-
Chondrus verrucosus ?
-
?
GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
-
Chondrus verrucosus ?
-
?
GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
-
Chondrus verrucosus ?
-
?
GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O substrate of isozymes ChiA, ChiB, and ChiC with formation of (N-acetylglucosamine)n and n = 2 to 4 Chondrus verrucosus ?
-
?
GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O substrate of isozymes ChiA, ChiB, and ChiC with formation of (N-acetylglucosamine)n and n = 2 to 4 Chondrus verrucosus ?
-
?
additional information isozymes ChiA, ChiB, and ChiC degrade squid pen beta-chitin to a higher degree than shrimp shell or crab shell alpha-chitin, isozyme specificity towards soluble substrates, overview. No activity with 4-nitrophenyl-(GlcNAc)3. General sctivity of isozymes in descending order: ChiA, ChiC, ChiB Chondrus verrucosus ?
-
?

Subunits

Subunits Comment Organism
? x * 24500, isozymes ChiA and ChiC, SDS-PAGE, x * 25500, isozyme ChiB, SDS-PAGE Chondrus verrucosus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
isozyme ChiB Chondrus verrucosus
80
-
isozymes ChiA and ChiC Chondrus verrucosus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
additional information
-
isozyme temperature profiles, overview Chondrus verrucosus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
1
-
isozyme ChiB Chondrus verrucosus
2
-
isozymes ChiA and ChiC Chondrus verrucosus

pH Range

pH Minimum pH Maximum Comment Organism
additional information
-
isozyme pH profiles, overview Chondrus verrucosus

pI Value

Organism Comment pI Value Maximum pI Value
Chondrus verrucosus isozymes ChiA and ChiC
-
3.5
Chondrus verrucosus isozyme ChiB
-
4.6