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Literature summary for 3.2.1.14 extracted from

  • Lin, F.P.; Chuang, H.H.; Liu, Y.H.; Hsieh, C.Y.; Lin, P.W.; Lin, H.Y.
    Effects of C-terminal amino acids truncation on enzyme properties of Aeromonas caviae D1 chitinase (2009), Arch. Microbiol., 191, 265-273.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type and truncation mutant enzymes in Escherichia coli BL21(DE3) Aeromonas caviae

Protein Variants

Protein Variants Comment Organism
additional information generation of truncated mutant AcD1Chi-AK606 Aeromonas caviae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.62
-
4-methylumbelliferyl-GlcNAcbeta(1->4)GlcNAc pH 6.0, 50°C, recombinant His-tagged truncation mutant enzyme Aeromonas caviae
5.35
-
4-methylumbelliferyl-GlcNAcbeta(1->4)GlcNAc pH 6.0, 50°C, recombinant His-tagged wild-type enzyme Aeromonas caviae
53.33
-
4-methylumbelliferyl GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc pH 6.0, 50°C, recombinant His-tagged wild-type enzyme Aeromonas caviae
57.83
-
4-methylumbelliferyl GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc pH 6.0, 50°C, recombinant His-tagged truncation mutant enzyme Aeromonas caviae

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Aeromonas caviae
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
66000
-
x * 97000, recombinant wild-type enzyme, SDS-PAGE, x * 66000, recombinant truncation mutant enzyme, SDS-PAGE Aeromonas caviae
97000
-
x * 97000, recombinant wild-type enzyme, SDS-PAGE, x * 66000, recombinant truncation mutant enzyme, SDS-PAGE Aeromonas caviae

Organism

Organism UniProt Comment Textmining
Aeromonas caviae
-
-
-
Aeromonas caviae D1
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
cell culture the organism produces an extracellular chitinase when grown on the chitin as a sole carbon source Aeromonas caviae
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl GlcNAcbeta(1-4)GlcNAc + H2O
-
Aeromonas caviae ?
-
?
4-methylumbelliferyl GlcNAcbeta(1-4)GlcNAc + H2O
-
Aeromonas caviae D1 ?
-
?
4-methylumbelliferyl GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
-
Aeromonas caviae ?
-
?
4-methylumbelliferyl GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
-
Aeromonas caviae D1 ?
-
?
alpha-chitin + H2O insoluble substrate Aeromonas caviae ?
-
?
alpha-chitin + H2O insoluble substrate Aeromonas caviae D1 ?
-
?
GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
-
Aeromonas caviae ?
-
?
GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
-
Aeromonas caviae D1 ?
-
?

Subunits

Subunits Comment Organism
? x * 97000, recombinant wild-type enzyme, SDS-PAGE, x * 66000, recombinant truncation mutant enzyme, SDS-PAGE Aeromonas caviae

Synonyms

Synonyms Comment Organism
AcD1ChiA
-
Aeromonas caviae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Aeromonas caviae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0007
-
4-methylumbelliferyl GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc pH 6.0, 50°C, recombinant His-tagged wild-type enzyme Aeromonas caviae
0.0012
-
4-methylumbelliferyl GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc pH 6.0, 50°C, recombinant His-tagged truncation mutant enzyme Aeromonas caviae
0.0047
-
4-methylumbelliferyl-GlcNAcbeta(1->4)GlcNAc pH 6.0, 50°C, recombinant His-tagged truncation mutant enzyme Aeromonas caviae
0.006
-
4-methylumbelliferyl-GlcNAcbeta(1->4)GlcNAc pH 6.0, 50°C, recombinant His-tagged wild-type enzyme Aeromonas caviae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
Aeromonas caviae