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Literature summary for 3.2.1.14 extracted from
- Molecular and biochemical characterization of an endochitinase (ChiA-HD73) from Bacillus thuringiensis subsp. kurstaki HD-73 (2007), Mol. Biotechnol., 39, 29-37.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli DH5alphaF' | Bacillus thuringiensis |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 74500 | - |
x * 74500, calculated from seuence | Bacillus thuringiensis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus thuringiensis | A5YRG4 | subsp. kurstaki HD-73 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Bacillus thuringiensis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-methylumbelliferyl-N,N',N''-triacetylchitotriose + H2O | - |
Bacillus thuringiensis | ? | - |
? |  |
| 4-methylumbelliferyl-N,N'-diacetylchitobiose + H2O | - |
Bacillus thuringiensis | ? | - |
? | |
| additional information | no activity with 4-methylumbelliferyl-(GlcNAc) | Bacillus thuringiensis | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 74500, calculated from seuence | Bacillus thuringiensis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ChiA-HD73 | - |
Bacillus thuringiensis |
| endochitinase | - |
Bacillus thuringiensis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
- |
Bacillus thuringiensis |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 70 | 30°C: about 65% of maximal activity, 70°C: about 45% of maximal activity | Bacillus thuringiensis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
- |
Bacillus thuringiensis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 8 | pH 4.5: about 55% of maximal activity, pH 8.0: about 65% of maximal activity | Bacillus thuringiensis |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Bacillus thuringiensis | calculated from sequence | - |
5.75 |
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