Literature summary for 3.2.1.14 extracted from

Sasaki, C.; Varum, K.M.; Itoh, Y.; Tamoi, M.; Fukamizo, T.
Rice chitinases: sugar recognition specificities of the individual subsites (2006), Glycobiology, 16, 1242-1250.

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Cloned(Commentary)

Cloned (Comment)	Organism
the chitinase gene containing His-tag is expressed in Pichia pastoris	Oryza sativa

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
additional information	Oryza sativa	the class III enzyme OsChib1a might act toward an endogenous complex carbohydrate containing GlcNAc residue	?	-	?
additional information	Oryza sativa	the target of the class I enzyme OsChia1cDELTAChBD might be a consecutive GlcNAc sequence probably in the cell wall of fungal pathogen	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Oryza sativa	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	the class III enzyme OsChib1a might act toward an endogenous complex carbohydrate containing GlcNAc residue	Oryza sativa	?	-	?
additional information	the target of the class I enzyme OsChia1cDELTAChBD might be a consecutive GlcNAc sequence probably in the cell wall of fungal pathogen	Oryza sativa	?	-	?
partially N-acetylated chitosan + H2O	for the class I enzyme OsChia1cDELTAChBD, the reducing end residue is preferentially acetylated, although the specificity is not absolute. The nearest neighbor to the acetylated reducing end residue is specifically acetylated. The nonreducing end residue produced by the class I enzyme is exclusively acetylated, although there is a low but significant preference for deacetylated units at the nearest neighbor to the nonreducing end. The three contiguous subsites (-2), (-1), and (+1) of the class I enzyme are specific to three consecutive GlcNAc residues of the substrate	Oryza sativa	?	-	?
partially N-acetylated chitosan + H2O	the reducing end residue of the enzymatic products obtained by the class III enzyme OsChib1a is exclusively acetylated, whereas both acetylated and deacetylated units are found at the nearest neighbor to the reducing end residue. Both acetylated and deacetylated units are also found at the nonreducing end residue and its nearest neighbor of the class III enzyme products. Only subsite (-1) among the contiguous subsites (-2) to (+2) of the class III enzyme is specific to an acetylated residue	Oryza sativa	?	-	?

Synonyms

Synonyms	Comment	Organism
OsChia1cDELTAChBD	-	Oryza sativa
OsChib1a	-	Oryza sativa

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Release 2023.1 (January 2023)