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Literature summary for 3.2.1.14 extracted from

  • Molinari, L.M.; Pedroso, R.B.; Scoaris, D.d.; Ueda-Nakamura, T.; Nakamura, C.V.; Dias Filho, B.P.
    Identification and partial characterization of a chitinase from Nile tilapia, Oreochromis niloticus (2007), Comp. Biochem. Physiol. B, 146, 81-87.
    View publication on PubMed
Search for more literature for 3.2.1.14

Inhibitors

Inhibitors Comment Organism Structure
Ag2+ 1 mM, complete inhibition of hydrolysis of 4-methylumbelliferyl-N,N'-diacetylchitobiose and 4-methylumbelliferyl-N,N',N''-triacetylchitotriose Oreochromis niloticus
CaCl2 1 mM, 27% inhibition of activity with 4-methylumbelliferyl-N,N',N''-triacetylchitotriose, no inhibition of activity with 4-methylumbelliferyl-(GlcNAc)2 Oreochromis niloticus
EDTA 1 mM, complete inhibition of hydrolysis of 4-methylumbelliferyl-N,N',N''-triacetylchitotriose, 79% loss of activity with 4-methylumbelliferyl-(GlcNAc)2 Oreochromis niloticus
Hg2+ 1 mM, complete inhibition of hydrolysis of 4-methylumbelliferyl-N,N'-diacetylchitobiose and 4-methylumbelliferyl-N,N',N''-triacetylchitotriose Oreochromis niloticus
Mg2+ 1 mM, complete inhibition of hydrolysis of 4-methylumbelliferyl-N,N',N''-triacetylchitotriose, 97% loss of activity with 4-methylumbelliferyl-(GlcNAc)2 Oreochromis niloticus
Mn2+ 1 mM, complete inhibition of hydrolysis of 4-methylumbelliferyl-N,N'-diacetylchitobiose and 4-methylumbelliferyl-N,N',N''-triacetylchitotriose Oreochromis niloticus
NaCl 500 mM, 34% inhibition of activity with 4-methylumbelliferyl-N,N',N''-triacetylchitotriose, no inhibition of activity with 4-methylumbelliferyl-(GlcNAc)2 Oreochromis niloticus
ZnSO4 1 mM, 37% inhibition of activity with 4-methylumbelliferyl-(GlcNAc)2, no inhibition of activity with 4-methylumbelliferyl-N,N',N''-triacetylchitotriose Oreochromis niloticus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
75000
-
 1 * 75000, SDS-PAGE Oreochromis niloticus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Oreochromis niloticus the enzyme may play a role in digestion and defense ?
-
 ?

Organism

Organism UniProt Comment Textmining
Oreochromis niloticus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
blood serum
-
 Oreochromis niloticus
-
intestine
-
 Oreochromis niloticus
-
stomach
-
 Oreochromis niloticus
-

Storage Stability

Storage Stability Organism
-20°C, stable Oreochromis niloticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl-N,N',N''-triacetylchitotriose + H2O activity with 4-methylumbelliferyl-N,N'-diacetylchitobiose is higher than activity with 4-methylumbelliferyl-N,N',N''-triacetylchitotriose Oreochromis niloticus ?
-
 ?
4-methylumbelliferyl-N,N'-diacetylchitobiose + H2O activity with 4-methylumbelliferyl-N,N'-diacetylchitobiose is higher than activity with 4-methylumbelliferyl-N,N',N''-triacetylchitotriose Oreochromis niloticus ?
-
 ?
additional information the enzyme may play a role in digestion and defense Oreochromis niloticus ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 75000, SDS-PAGE Oreochromis niloticus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
20
-
 substrate: 4-methylumbelliferyl-(GlcNAc)2 Oreochromis niloticus
50
-
 substrate: 4-methylumbelliferyl-N,N',N''-triacetylchitotriose Oreochromis niloticus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
10 40 10°C: 40% of maximal activity, 40°C: 80% of maximal activity, substrate: 4-methylumbelliferyl-(GlcNAc)2 Oreochromis niloticus
20 50 20°C: 20% of maximal activity, 50°C: maximal activity, substrate: 4-methylumbelliferyl-N,N',N''-triacetylchitotriose Oreochromis niloticus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 substrate: 4-methylumbelliferyl-(GlcNAc)2 Oreochromis niloticus
9
-
 substrate: 4-methylumbelliferyl-N,N',N''-triacetylchitotriose Oreochromis niloticus

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 8 the enzyme loses its activity below pH 5.0 and above pH 8.0 Oreochromis niloticus