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Literature summary for 3.2.1.14 extracted from

  • Pantoom, S.; Songsiriritthigul, C.; Suginta, W.
    The effects of the surface-exposed residues on the binding and hydrolytic activities of Vibrio carchariae chitinase A (2008), BMC Biochem., 9, 2.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.14

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type and mutant enzymes in Escherichia coli Vibrio harveyi

Protein Variants

Protein Variants Comment Organism
S33W strong increase in binding activity towards crystalline alpha-chitin and colloidal chitin. Mutation has no effect on specific hydrolyzing activity against p-nitrophenyl-N,N'-diacetylchitobiose, or the catalytic efficiency against chitohexaose Vibrio harveyi
W231A strong increase in binding activity towards crystalline alpha-chitin and colloidal chitin. Mutant displayed reduced hydrolytic activity. Mutation has no effect on specific hydrolyzing activity against p-nitrophenyl-N,N'-diacetylchitobiose, or the catalytic efficiency against chitohexaose Vibrio harveyi
W70A strong decrease in binding activity towards crystalline alpha-chitin and colloidal chitin. Mutant enzyme shows severe loss in hydrolytic activities. Mutation has no effect on specific hydrolyzing activity against p-nitrophenyl-N,N'-diacetylchitobiose, or the catalytic efficiency against chitohexaose Vibrio harveyi
Y245W strong decrease in binding activity towards crystalline alpha-chitin and colloidal chitin. Mutant displayes enhanced hydrolytic activity. Mutation has no effect on specific hydrolyzing activity against p-nitrophenyl-N,N'-diacetylchitobiose, or the catalytic efficiency against chitohexaose Vibrio harveyi

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.163
-
 chitohexaose pH 5.5, 37°C, mutant enzyme W231F Vibrio harveyi
0.171
-
 chitohexaose pH 5.5, 37°C, mutant enzyme S33A Vibrio harveyi
0.185
-
 chitohexaose pH 5.5, 37°C, mutant enzyme W70A Vibrio harveyi
0.189
-
 chitohexaose pH 5.5, 37°C, mutant enzyme W231A Vibrio harveyi
0.201
-
 chitohexaose pH 5.5, 37°C, mutant enzyme Y245W Vibrio harveyi
0.21
-
 chitohexaose pH 5.5, 37°C, mutant enzyme S33W Vibrio harveyi
0.218
-
 chitohexaose pH 5.5, 37°C, wild-type enzyme Vibrio harveyi

Organism

Organism UniProt Comment Textmining
Vibrio harveyi
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Vibrio harveyi

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chitin + H2O low activity with crystallin chitin, high activity with colloidal chitin Vibrio harveyi ?
-
 ?
chitohexaose + H2O
-
 Vibrio harveyi ?
-
 ?
p-nitrophenyl-N,N'-diacetylchitobiose + H2O
-
 Vibrio harveyi p-nitrophenol + N,N'-diacetylchitobiose
-
 ?

Synonyms

Synonyms Comment Organism
chitinase A
-
 Vibrio harveyi

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.4
-
 chitin pH 5.5, 37°C, colloidal chitin, mutant enzym W70A Vibrio harveyi
0.6
-
 chitin pH 5.5, 37°C, colloidal chitin, mutant enzym W231A Vibrio harveyi
0.9
-
 chitin pH 5.5, 37°C, colloidal chitin, mutant enzym S33A Vibrio harveyi
0.9
-
 chitin pH 5.5, 37°C, colloidal chitin, mutant enzym W231F Vibrio harveyi
1.2
-
 chitin pH 5.5, 37°C, colloidal chitin, wild-type enzyme Vibrio harveyi
1.4
-
 chitin pH 5.5, 37°C, colloidal chitin, mutant enzym S33W Vibrio harveyi
1.7
-
 chitin pH 5.5, 37°C, colloidal chitin, mutant enzym Y245W Vibrio harveyi
2.4
-
 chitohexaose pH 5.5, 37°C, mutant enzyme W231F Vibrio harveyi
2.5
-
 chitohexaose pH 5.5, 37°C, mutant enzyme W70A Vibrio harveyi
2.6
-
 chitohexaose pH 5.5, 37°C, mutant enzyme S33A Vibrio harveyi
2.6
-
 chitohexaose pH 5.5, 37°C, mutant enzyme W231A Vibrio harveyi
2.6
-
 chitohexaose pH 5.5, 37°C, mutant enzyme Y245W Vibrio harveyi
2.8
-
 chitohexaose pH 5.5, 37°C, mutant enzyme S33W Vibrio harveyi
2.9
-
 chitohexaose pH 5.5, 37°C, wild-type enzyme Vibrio harveyi