Cloned (Comment) | Organism |
---|---|
- |
Vibrio harveyi |
Protein Variants | Comment | Organism |
---|---|---|
D392N | mutation completely abolishes hydrolyzing activity against colloidal chitin and greatly reduces the hydrolyzing activity against the p-nitrophenyl-N,N'-diacetylchitobiose. Km-value for p-nitrophenyl-N,N'-diacetylchitobiose is 4fold lower than wild-type value, turnover-number is 6.9fold lower than wild-type value | Vibrio harveyi |
W168G | mutation completely abolishes hydrolyzing activity against colloidal chitin and greatly reduces the hydrolyzing activity against the p-nitrophenyl-N,N'-diacetylchitobiose. Km-value for p-nitrophenyl-N,N'-diacetylchitobiose is nearly identical to wild-type value, turnover-number is 19.5fold lower than wild-type value | Vibrio harveyi |
W275G | Km-value for p-nitrophenyl-N,N'-diacetylchitobiose is 4.6fold lower than wild-type value, turnover-number is 18fold lower than wild-type value | Vibrio harveyi |
W397F | mutation enhances hydrolyzing activity towards p-nitrophenyl-N,N'-diacetylchitobiose to 142% and slightly increases activity towards colloidal chitin to 102.5%. Km-value for p-nitrophenyl-N,N'-diacetylchitobiose is 1.16fold higher than wild-type value, turnover-number is 1.2fold higher than wild-type value | Vibrio harveyi |
W570G | mutation completely abolishes hydrolyzing activity against colloidal chitin and greatly reduces the hydrolyzing activity against the p-nitrophenyl-N,N'-diacetylchitobiose (5% of wild-type activity) | Vibrio harveyi |
Y171G | mutation completely abolishes hydrolyzing activity against colloidal chitin and greatly reduces the hydrolyzing activity against the p-nitrophenyl-N,N'-diacetylchitobiose. Km-value for p-nitrophenyl-N,N'-diacetylchitobiose is 2.5fold lower than wild-type value, turnover-number is 9.7fold lower than wild-type value | Vibrio harveyi |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.062 | - |
p-nitrophenyl-N,N'-diacetylchitobiose | pH 5.0, 30°C, mutant enzyme W275G | Vibrio harveyi | |
0.071 | - |
p-nitrophenyl-N,N'-diacetylchitobiose | pH 5.0, 30°C, mutant enzyme D392N | Vibrio harveyi | |
0.115 | - |
p-nitrophenyl-N,N'-diacetylchitobiose | pH 5.0, 30°C, mutant enzyme Y171G | Vibrio harveyi | |
0.278 | - |
p-nitrophenyl-N,N'-diacetylchitobiose | pH 5.0, 30°C, mutant enzyme W168G | Vibrio harveyi | |
0.288 | - |
p-nitrophenyl-N,N'-diacetylchitobiose | pH 5.0, 30°C, wild-type enzyme | Vibrio harveyi | |
0.315 | - |
p-nitrophenyl-N,N'-diacetylchitobiose | pH 5.0, 30°C, mutant enzyme W397F | Vibrio harveyi |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vibrio harveyi | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Vibrio harveyi |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
chitin + H2O | - |
Vibrio harveyi | ? | - |
? | |
N,N',N'',N''',N'''',N'''''-hexaacetylchitohexaose + H2O | the time course of hydrolysis exhibits distinct hydrolytic patterns between wild-type and mutants W275G and W397F. Wild-type enzyme initially hydrolyzes N,N',N'',N''',N'''',N'''''-hexaacetylchitohexaose to N,N',N'',N'''-tetraacetylchitotetraose and N,N'-diacetylchitobiose, and finally N,N'-diacetylchitobiose is formed as a major end product. W275G primarily creates N,N'-diacetylchitobiose to N,N',N'',N''',N''''-pentaacetylchitopentaose intermediates, and later N,N'-diacetylchitobiose and N,N',N''-triacetylchitotriose are formed as stable products. W397F initially produces (GlcNAc)1 to N,N',N'',N''',N''''-pentaacetylchitopentaose, and then the N,N',N''-triacetylchitotriose to N,N',N'',N''',N''''-pentaacetylchitopentaose are broken down to (GlcNAc)1 and N,N'-diacetylchitobiose end products | Vibrio harveyi | ? | - |
? | |
p-nitrophenyl-N,N'-diacetylchitobiose + H2O | - |
Vibrio harveyi | p-nitrophenol + N,N'-diacetylchitobiose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
chitinase A | - |
Vibrio harveyi |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.11 | - |
p-nitrophenyl-N,N'-diacetylchitobiose | pH 5.0, 30°C, mutant enzyme W168G | Vibrio harveyi | |
0.12 | - |
p-nitrophenyl-N,N'-diacetylchitobiose | pH 5.0, 30°C, mutant enzyme W275G | Vibrio harveyi | |
0.21 | - |
p-nitrophenyl-N,N'-diacetylchitobiose | pH 5.0, 30°C, mutant enzyme Y171G | Vibrio harveyi | |
0.31 | - |
p-nitrophenyl-N,N'-diacetylchitobiose | pH 5.0, 30°C, mutant enzyme D392N | Vibrio harveyi | |
2.15 | - |
p-nitrophenyl-N,N'-diacetylchitobiose | pH 5.0, 30°C, wild-type enzyme | Vibrio harveyi | |
2.59 | - |
p-nitrophenyl-N,N'-diacetylchitobiose | pH 5.0, 30°C, mutant enzyme W397F | Vibrio harveyi |