Cloned (Comment) | Organism |
---|---|
the recombinant VpChiA and its C-terminal truncated G589 mutantVpChiAG589 encoded in 1881 and 1791 bp DNA fragments, respectively, are expressed in Escherichia coli using the pET-20b(+) expression system | Vibrio parahaemolyticus |
Protein Variants | Comment | Organism |
---|---|---|
G598X | C-terminal truncated G589 mutantVpChiAG589 shows higher temperature stability than recombinant VpChiA. kcat for 4-methylumbelliferyl-N,N'-diacetylchitobiose is 1.16fold higher than wild-type value, kcat for 4-methylumbelliferyl-N,N',N''-triacetylchitotriose is 1.8fold lower than wild-type value | Vibrio parahaemolyticus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 1 mM, 22% inhibition of VpChiA, 15% inhibition of mutant enzyme VpChiAG589 | Vibrio parahaemolyticus | |
Cu2+ | 1 mM, 38% inhibition of VpChiA, 81% inhibition of mutant enzyme VpChiAG589 | Vibrio parahaemolyticus | |
EDTA | 10 mM, 21% inhibition of VpChiA, 17% inhibition of mutant enzyme VpChiAG589 | Vibrio parahaemolyticus | |
Fe2+ | 1 mM, abolishes the activities of both VpChiA and mutant enzyme VpChiAG589 | Vibrio parahaemolyticus | |
Fe3+ | 1 mM, abolishes the activities of both VpChiA and mutant enzyme VpChiAG589 | Vibrio parahaemolyticus | |
SDS | 5%, abolishes the activities of both VpChiA and mutant enzyme VpChiAG589 | Vibrio parahaemolyticus | |
Triton X-100 | 5%, 31% inhibition of VpChiA and mutant enzyme VpChiAG589 | Vibrio parahaemolyticus | |
Urea | 4 mM, 96% inhibition of VpChiA and mutant enzyme VpChiAG589 | Vibrio parahaemolyticus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00144 | - |
4-methylumbelliferyl-N,N',N''-triacetylchitotriose | pH 7, 37°C, C-terminal truncated G589 mutant enzyme VpChiAG589 | Vibrio parahaemolyticus | |
0.00261 | - |
4-methylumbelliferyl-N,N',N''-triacetylchitotriose | pH 7, 37°C, VpChiA | Vibrio parahaemolyticus | |
0.00775 | - |
4-methylumbelliferyl-N,N'-diacetylchitobiose | pH 7, 37°C, VpChiA | Vibrio parahaemolyticus | |
0.01172 | - |
4-methylumbelliferyl-N,N'-diacetylchitobiose | pH 7, 37°C, C-terminal truncated G589 mutant enzyme VpChiAG589 | Vibrio parahaemolyticus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vibrio parahaemolyticus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(GlcNAc)7 + H2O | - |
Vibrio parahaemolyticus | ? | - |
? | |
4-methylumbelliferyl-N,N',N''-triacetylchitotriose + H2O | - |
Vibrio parahaemolyticus | ? | - |
? | |
4-methylumbelliferyl-N,N'-diacetylchitobiose + H2O | - |
Vibrio parahaemolyticus | ? | - |
? | |
alpha-chitin + H2O | - |
Vibrio parahaemolyticus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
VpChiA | - |
Vibrio parahaemolyticus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
substrate: 4-methylumbelliferyl-N,N'-diacetylchitobiose or 4-methylumbelliferyl-N,N',N''-triacetylchitotriose, VpChiA and C-terminal truncated G589 mutant enzyme VpChiAG589 | Vibrio parahaemolyticus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 50 | 25°C: about 25% of maximal activity, VpChiA and C-terminal truncated G589 mutant enzyme VpChiAG589. 50°C: 70% of maximal activity, VpChiA. 90% of maximal activity, C-terminal truncated G589 mutant enzyme VpChiAG589 | Vibrio parahaemolyticus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
30 min, 25% loss of activity of VpChiA and 5% loss of activity of C-terminal truncated G589 mutant enzyme VpChiAG589 | Vibrio parahaemolyticus |
80 | - |
30 min, 55% loss of activity of VpChiA and 20% loss of activity of C-terminal truncated G589 mutant enzyme VpChiAG589 | Vibrio parahaemolyticus |
90 | - |
30 min, 70% loss of activity of VpChiA and 60% loss of activity of C-terminal truncated G589 mutant enzyme VpChiAG589 | Vibrio parahaemolyticus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.52 | - |
4-methylumbelliferyl-N,N'-diacetylchitobiose | pH 7, 37°C, VpChiA | Vibrio parahaemolyticus | |
0.833 | - |
4-methylumbelliferyl-N,N'-diacetylchitobiose | pH 7, 37°C, mutant enzyme VpChiAG589 | Vibrio parahaemolyticus | |
13.94 | - |
4-methylumbelliferyl-N,N'-diacetylchitobiose | pH 7, 37°C, VpChiA | Vibrio parahaemolyticus | |
16.16 | - |
4-methylumbelliferyl-N,N'-diacetylchitobiose | pH 7, 37°C, mutant enzyme VpChiAG589 | Vibrio parahaemolyticus | |
21 | - |
4-methylumbelliferyl-N,N',N''-triacetylchitotriose | pH 7, 37°C, VpChiA | Vibrio parahaemolyticus | |
68.87 | - |
4-methylumbelliferyl-N,N',N''-triacetylchitotriose | pH 7, 37°C, mutant enzyme VpChiAG589 | Vibrio parahaemolyticus | |
110.6 | - |
4-methylumbelliferyl-N,N',N''-triacetylchitotriose | pH 7, 37°C, VpChiA | Vibrio parahaemolyticus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
substrate: 4-methylumbelliferyl-N,N'-diacetylchitobiose or 4-methylumbelliferyl-N,N',N''-triacetylchitotriose, VpChiA and C-terminal truncated G589 mutant enzyme VpChiAG589 | Vibrio parahaemolyticus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 8 | pH 5.0: about 90% of maximal activity, pH 8.0: about 60% of maximal activity, VpChiA and C-terminal truncated G589 mutant enzyme VpChiAG589 | Vibrio parahaemolyticus |