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Literature summary for 3.2.1.14 extracted from

  • Morimoto, K.; Yoshimoto, M.; Karita, S.; Kimura, T.; Ohmiya, K.; Sakka, K.
    Characterization of the third chitinase Chi18C of Clostridium paraputrificum M-21 (2007), Appl. Microbiol. Biotechnol., 73, 1106-1113.
    View publication on PubMed
Search for more literature for 3.2.1.14

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00044
-
 4-methylumbelliferyl-beta-D-N,N',N''-triacetylchitotriose
-
 Clostridium paraputrificum

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Clostridium paraputrificum
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
74651
-
 x * 74651, calculated from sequence Clostridium paraputrificum

Organism

Organism UniProt Comment Textmining
Clostridium paraputrificum Q75ZW9
-
-
Clostridium paraputrificum M-21 Q75ZW9
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Clostridium paraputrificum

Source Tissue

Source Tissue Comment Organism Textmining
culture medium expression of chi18C is inducible with chitinous substrates Clostridium paraputrificum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
26.6
-
-
 Clostridium paraputrificum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl-beta-D-N,N',N''-triacetylchitotriose + H2O
-
 Clostridium paraputrificum ?
-
 ?
4-methylumbelliferyl-beta-D-N,N',N''-triacetylchitotriose + H2O
-
 Clostridium paraputrificum M-21 ?
-
 ?
chitin + H2O active toward soluble and insoluble chitin preparations Clostridium paraputrificum ?
-
 ?
chitin + H2O active toward soluble and insoluble chitin preparations Clostridium paraputrificum M-21 ?
-
 ?
additional information not active toward 4-methylumbelliferyl-N-acetylglucosamine or 4-methylumbelliferyl-beta-D-N-N'-diacetylchitobioside Clostridium paraputrificum ?
-
 ?
additional information not active toward 4-methylumbelliferyl-N-acetylglucosamine or 4-methylumbelliferyl-beta-D-N-N'-diacetylchitobioside Clostridium paraputrificum M-21 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 74651, calculated from sequence Clostridium paraputrificum

Synonyms

Synonyms Comment Organism
Chi18C
-
 Clostridium paraputrificum
chitinase 18C
-
 Clostridium paraputrificum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Clostridium paraputrificum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Clostridium paraputrificum