Literature summary for 3.2.1.14 extracted from

Daimon, T.; Katsuma, S.; Iwanaga, M.; Kang, W.; Shimada, T.
The BmChi-h gene, a bacterial-type chitinase gene of Bombyx mori, encodes a functional exochitinase that plays a role in the chitin degradation during the molting process (2005), Insect Biochem. Mol. Biol., 35, 1112-1123.

Search for more literature for 3.2.1.14

Cloned(Commentary)

Cloned (Comment)	Organism
DNA and amino acid sequence determination and analysis, phylogenetic analysis	Antheraea pernyi
DNA and amino acid sequence determination and analysis, phylogenetic analysis	Ostrinia furnacalis
DNA and amino acid sequence determination and analysis, phylogenetic analysis	Agrius convolvuli
DNA and amino acid sequence determination and analysis, phylogenetic analysis	Mythimna separata
DNA and amino acid sequence determination and analysis, phylogenetic analysis	Samia cynthia
gene Chi-h, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of His-tagged wild-type and mutant Chi-h in Spodoptera frugiperda Sf9 cells using the Autographa californica nucleopolyhedrovirus for transfection constructed in a Bac-to-Bac system	Bombyx mori

Protein Variants

Protein Variants	Comment	Organism
D306N	site-directed mutagenesis using different sets of primers, the mutant enzyme shows reduced activity and high pH sensitivity compared to the wild-type enzyme	Bombyx mori
D306N/D310Q	site-directed mutagenesis using different sets of primers, inactive mutant	Bombyx mori
D310Q	site-directed mutagenesis using different sets of primers, inactive mutant	Bombyx mori

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
additional information	Bombyx mori	Chi-h plays a role in the chitin degradation during the molting process	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Agrius convolvuli	Q4AE58	-	-
Antheraea pernyi	Q4AE61	-	-
Bombyx mori	Q869E2	gene Chi-h encodes an exochitinase, the organism also contains an endochitinase	-
Mythimna separata	Q4AE57	i.e. Mythimna separata	-
Ostrinia furnacalis	Q4AE59	-	-
Samia cynthia	Q4AE60	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His-tagged wild-type and mutant Chi-h from Sf9 insect cells by metal affinity chromatography and dialysis	Bombyx mori

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-methylumbelliferyl beta-N,N',N''-triacetylchitotrioside + H2O	-	Bombyx mori	4-methylumbelliferol + beta-N,N',N''-triacetylchitotriose	-	?
4-methylumbelliferyl beta-N,N'-diacetylchitobioside + H2O	-	Bombyx mori	4-methylumbelliferol + beta-N,N'-diacetylchitobiose	-	?
4-methylumbelliferyl beta-N-acetyl-D-glucosamine + H2O	-	Bombyx mori	4-methylumbelliferol + beta-N-acetyl-D-glucosamine	-	?
chitin oligosaccharides + H2O	glycol chitin assay method, (GlcNAc)1-6	Bombyx mori	N-acetyl glucosamine	-	?
additional information	Chi-h plays a role in the chitin degradation during the molting process	Bombyx mori	?	-	?

Synonyms

Synonyms	Comment	Organism
bacterial-type chitinase	-	Bombyx mori
Chi-h	-	Antheraea pernyi
Chi-h	-	Ostrinia furnacalis
Chi-h	-	Agrius convolvuli
Chi-h	-	Mythimna separata
Chi-h	-	Bombyx mori
Chi-h	-	Samia cynthia
exochitinase	-	Bombyx mori

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
25	-	assay at, with fluorescent substrates	Bombyx mori
37	-	assay at, with chitin substrates	Bombyx mori

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
4	-	exochitinase mutant D306N	Bombyx mori
6.2	-	wild-type exochitinase	Bombyx mori

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Release 2023.1 (January 2023)