Any feedback?
Literature summary for 3.2.1.14 extracted from
- Isolation and identification of Streptomyces fradiae SU-1 from Thailand and protoplast transformation with the chitinase B gene from Nocardiopsis prasina OPC-131 (2005), Curr. Microbiol., 51, 116-121.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene chiB337, functional expression in Streptomyces fradiae strain SU-1 using the Escherichia coli-Streptomyces shuttle vector pFIS318 for transformation of protoplasts, secretion of chitinases by the recombinant strain, subcloning in Escherichia coli strain JM109 | Nocardiopsis prasina |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | two extracellular isozymes are secreted to the culture medium | Streptomyces fradiae | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 26000 | - |
x * 26000, isozyme 1, SDS-PAGE, x * 43000, isozyme 2, SDS-PAGE | Streptomyces fradiae |
| 31500 | - |
x * 31500, recombinant enzyme, SDS-PAGE | Nocardiopsis prasina |
| 43000 | - |
x * 26000, isozyme 1, SDS-PAGE, x * 43000, isozyme 2, SDS-PAGE | Streptomyces fradiae |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Nocardiopsis prasina | the enzyme shows antifungal activity against Fusarium moniliforme, overview | ? | - |
? |  |
| additional information | Nocardiopsis prasina OPC-131 | the enzyme shows antifungal activity against Fusarium moniliforme, overview | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Nocardiopsis prasina | - |
gene chiB337 | - |
| Nocardiopsis prasina OPC-131 | - |
gene chiB337 | - |
| Streptomyces fradiae | - |
two extracellular isozymes | - |
| Streptomyces fradiae SU-1 | - |
two extracellular isozymes | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Streptomyces fradiae |
| additional information | - |
- |
Nocardiopsis prasina |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-methylumbelliferyl beta-D-N,N'-diacetylchitobioside + H2O | - |
Streptomyces fradiae | ? | - |
? | |
| 4-methylumbelliferyl beta-D-N,N'-diacetylchitobioside + H2O | - |
Nocardiopsis prasina | ? | - |
? | |
| 4-methylumbelliferyl beta-D-N,N'-diacetylchitobioside + H2O | - |
Streptomyces fradiae SU-1 | ? | - |
? | |
| 4-methylumbelliferyl beta-D-N,N'-diacetylchitobioside + H2O | - |
Nocardiopsis prasina OPC-131 | ? | - |
? | |
| additional information | the enzyme shows antifungal activity against Fusarium moniliforme, overview | Nocardiopsis prasina | ? | - |
? | |
| additional information | the enzyme shows antifungal activity against Fusarium moniliforme, overview | Nocardiopsis prasina OPC-131 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 26000, isozyme 1, SDS-PAGE, x * 43000, isozyme 2, SDS-PAGE | Streptomyces fradiae |
| ? | x * 31500, recombinant enzyme, SDS-PAGE | Nocardiopsis prasina |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| chitinase B | - |
Nocardiopsis prasina |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
