Cloned (Comment) | Organism |
---|---|
gene chi46, DNA and amino acid sequence determination and analysis, multiple regulatory proteins are involved in wild-type Chi46 expression, expression in Escherichia coli | Trichoderma reesei |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | the enzyme is secreted to the culture medium, the enzyme sequence contains a pre-pro type signal peptide | Trichoderma reesei | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42265 | - |
x * 46000, SDS-PAGE, x * 42265, Chi46, amino acid sequence calculation | Trichoderma reesei |
46000 | - |
x * 46000, SDS-PAGE, x * 42265, Chi46, amino acid sequence calculation | Trichoderma reesei |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trichoderma reesei | - |
gene chi46 | - |
Trichoderma reesei PC-3-7 | - |
gene chi46 | - |
Purification (Comment) | Organism |
---|---|
native enzyme from the culture medium | Trichoderma reesei |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
chitopentaose + H2O = chitobiose + chitotriose | exo-type substrate cleavage mechanism | Trichoderma reesei |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mycelium | - |
Trichoderma reesei | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
chitin + H2O | colloidal and ethylene glycol chitin, exo-type cleavage mode | Trichoderma reesei | GlcNAc2 + ? | main product from insoluble colloidal chitin | ? | |
chitin + H2O | colloidal and ethylene glycol chitin, exo-type cleavage mode | Trichoderma reesei PC-3-7 | GlcNAc2 + ? | main product from insoluble colloidal chitin | ? | |
chitosan 7B + H2O | the activity increases with increasing acetylation of the substrate | Trichoderma reesei | ? | - |
? | |
chitosan 7B + H2O | the activity increases with increasing acetylation of the substrate | Trichoderma reesei PC-3-7 | ? | - |
? | |
additional information | multiple regulatory proteins are involved in Chi46 expression | Trichoderma reesei | ? | - |
? | |
additional information | multiple regulatory proteins are involved in Chi46 expression | Trichoderma reesei PC-3-7 | ? | - |
? | |
N-acetylchito-oligosaccharides + H2O | endo-type cleavage mode | Trichoderma reesei | ? | - |
? | |
N-acetylchito-oligosaccharides + H2O | endo-type cleavage mode | Trichoderma reesei PC-3-7 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 46000, SDS-PAGE, x * 42265, Chi46, amino acid sequence calculation | Trichoderma reesei |
More | the enzyme sequence contains a pre-pro type signal peptide | Trichoderma reesei |
Synonyms | Comment | Organism |
---|---|---|
Chi46 | - |
Trichoderma reesei |