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Literature summary for 3.2.1.14 extracted from

  • Ogawa, K.; Yoshida, N.; Kariya, K.; Ohnishi, C.; Ikeda, R.
    Purification and characterization of a novel chitinase from Burkholderia cepacia strain KH2 isolated from the bed log of Lentinus edodes, Shiitake mushroom (2002), J. Gen. Appl. Microbiol., 48, 25-33.
    View publication on PubMed
Search for more literature for 3.2.1.14

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Burkholderia cepacia
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34000
-
 x * 34000, SDS-PAGE Burkholderia cepacia

Organism

Organism UniProt Comment Textmining
Burkholderia cepacia
-
 KH2
-
Burkholderia cepacia KH2
-
 KH2
-

Purification (Commentary)

Purification (Comment) Organism
-
 Burkholderia cepacia

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
440000
-
 after Sephacryl S-100 HR P-II-1 purification step Burkholderia cepacia

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chitin + H2O colloidal and patially deacetylated chitin PC-100 Burkholderia cepacia ?
-
 ?
chitin + H2O colloidal and patially deacetylated chitin PC-100 Burkholderia cepacia KH2 ?
-
 ?
glycol chitin + H2O
-
 Burkholderia cepacia ?
-
 ?
glycol chitin + H2O
-
 Burkholderia cepacia KH2 ?
-
 ?
N,N',N'',N''',N'''',N'''''-hexaacetylchitohexaose + H2O
-
 Burkholderia cepacia N,N'-diacetylchitobiose + N-acetyl-D-glucosamine
-
 ?
N,N',N'',N''',N'''',N'''''-hexaacetylchitohexaose + H2O
-
 Burkholderia cepacia KH2 N,N'-diacetylchitobiose + N-acetyl-D-glucosamine
-
 ?
N,N',N'',N''',N''''-pentaacetylchitopentaose + H2O
-
 Burkholderia cepacia N,N'-diacetylchitobiose + N-acetyl-D-glucosamine
-
 ?
N,N',N'',N''',N''''-pentaacetylchitopentaose + H2O
-
 Burkholderia cepacia KH2 N,N'-diacetylchitobiose + N-acetyl-D-glucosamine
-
 ?
N,N',N'',N'''-tetraacetylchitotetraose + H2O
-
 Burkholderia cepacia N-acetyl-D-glucosamine + N,N'-diacetylchitobiose + N,N',N''-triacetylchitotriose + N,N',N'',N'''-tetraacetylchitotetraose + N,N',N'',N''',N''''-pentaacetylchitopentaose + N,N',N'',N''',N'''',N'''''-hexaacetylchitohexaose transglycosilation activity ?
N,N',N''-triacetylchitotriose + H2O
-
 Burkholderia cepacia N,N'-diacetylchitobiose + N-acetyl-D-glucosamine
-
 ?
partially deacetylated chitin + H2O higher activity toward chitosan 7B, a 62% deacetylated chitosan, than toward the highly deacetylated chitosan substrates: chitosan EL, 9B, 8B and 10 Burkholderia cepacia N,N'-diacetylchitobiose + N,N',N''-triacetylchitotriose + N,N',N'',N'''-tetraacetylchitotetraose + N,N',N'',N''',N''''-pentaacetylchitopentaose + N,N',N'',N''',N'''',N'''''-hexaacetylchitohexaose + N,N',N'',N''',N'''',N''''',N''''''-heptaacetylchitoheptaose
-
 ?
partially deacetylated chitin + H2O higher activity toward chitosan 7B, a 62% deacetylated chitosan, than toward the highly deacetylated chitosan substrates: chitosan EL, 9B, 8B and 10 Burkholderia cepacia KH2 N,N'-diacetylchitobiose + N,N',N''-triacetylchitotriose + N,N',N'',N'''-tetraacetylchitotetraose + N,N',N'',N''',N''''-pentaacetylchitopentaose + N,N',N'',N''',N'''',N'''''-hexaacetylchitohexaose + N,N',N'',N''',N'''',N''''',N''''''-heptaacetylchitoheptaose
-
 ?

Subunits

Subunits Comment Organism
? x * 34000, SDS-PAGE Burkholderia cepacia

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Burkholderia cepacia

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
 50% activity after 3 h Burkholderia cepacia
65
-
 50% activity after 20 min Burkholderia cepacia

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
-
 Burkholderia cepacia

pH Range

pH Minimum pH Maximum Comment Organism
4 6
-
 Burkholderia cepacia

pI Value

Organism Comment pI Value Maximum pI Value
Burkholderia cepacia isoelectric focusing
-
 6