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Literature summary for 3.2.1.14 extracted from
- Different cleavage specificities of the dual catalytic domains in chitinase from the hyperthermophilic archaeon Thermococcus kodakaraensis KOD1 (2001), J. Biol. Chem., 276, 35629-35635.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| KCl | 0.2-1 M KCl increases activity of ChiADELTA4 and ChiADELTA4 to 110% | Thermococcus kodakarensis |  |
| NaCl | 0.2-1 M NaCl increases activity of ChiADELTA4 and ChiADELTA4 to 110% | Thermococcus kodakarensis | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression in Escherichia coli | Thermococcus kodakarensis |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| ChiADELTA2 | consits of chitin-binding domains ChBD2 and ChBD3 and catalytic domain B | Thermococcus kodakarensis |
| ChiADELTA3 | consits of chitin-binding domain ChBD1 and catalytic domain A | Thermococcus kodakarensis |
| ChiADELTA4 | consits only of catalytic domain B | Thermococcus kodakarensis |
| ChiADELTA5 | consits only of catalytic domain A | Thermococcus kodakarensis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermococcus kodakarensis | - |
KOD1 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Thermococcus kodakarensis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| chitin + H2O | - |
Thermococcus kodakarensis | N,N'-diacetylchitobiose + ? | major product | ? | |
| colloidal chitin + H2O | - |
Thermococcus kodakarensis | N,N'-diacetylchitobiose + ? | major product | ? | |
| ethylene glycolchitin + H2O | - |
Thermococcus kodakarensis | ? | - |
? | |
| N,N',N'',N''', N'''',N'''''-hexaacetylchitohexaose + 2 H2O | - |
Thermococcus kodakarensis | 3 N,N'-diacetylchitobiose | major product | ? | |
| N,N',N'',N''',N''''-pentaacetylchitopentaose + H2O | - |
Thermococcus kodakarensis | N,N'-diacetylchitobiose + N-acetyl-D-glucosamine | major product | ? | |
| N,N',N'',N'''-tetraacetylchitotetraose + H2O | - |
Thermococcus kodakarensis | 2 N,N'-diacetylchitobiose | major product | ? | |
| N,N',N''-triacetylchitotriose + H2O | - |
Thermococcus kodakarensis | N-acetyl-D-glucosamine + N,N'-diacetylchitobiose | - |
? | |
| partially N-acetylated chitosan + H2O | chitosan 7B, 8B, 9B and 10B | Thermococcus kodakarensis | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Tk-ChiA | - |
Thermococcus kodakarensis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 85 | - |
ChiADELTA4 | Thermococcus kodakarensis |
| 90 | - |
ChiADELTA5 | Thermococcus kodakarensis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
ChiADELTA4: over 60 min in a pH range from 5-9, ChiADELTA5: over 60 min in a pH range from 4-9 | Thermococcus kodakarensis |
| 90 | - |
half-life of ChiADELTA5 is 5 min | Thermococcus kodakarensis |
| 100 | - |
half-life of ChiADELTA4 is more than 7 h | Thermococcus kodakarensis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | - |
ChiADELTA4 | Thermococcus kodakarensis |
| 4.5 | 8 | ChiADELTA5 | Thermococcus kodakarensis |
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