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Literature summary for 3.2.1.14 extracted from

  • Xia, G.; Jin, C.; Zhou, J.; Yang, S.; Zhang, S.; Jin, C.
    A novel chitinase having a unique mode of action from Aspergillus fumigatus YJ-407 (2001), Eur. J. Biochem., 268, 4079-4085.
    View publication on PubMed
Search for more literature for 3.2.1.14

Inhibitors

Inhibitors Comment Organism Structure
Fe2+
-
 Aspergillus fumigatus
Hg2+
-
 Aspergillus fumigatus
Mn2+
-
 Aspergillus fumigatus
N-bromosuccinimide
-
 Aspergillus fumigatus
Pb2+
-
 Aspergillus fumigatus
Zn2+
-
 Aspergillus fumigatus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
46000
-
 x * 46000, SDS-PAGE Aspergillus fumigatus

Organism

Organism UniProt Comment Textmining
Aspergillus fumigatus
-
 YJ-407
-
Aspergillus fumigatus YJ-407
-
 YJ-407
-

Purification (Commentary)

Purification (Comment) Organism
-
 Aspergillus fumigatus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.36
-
-
 Aspergillus fumigatus

Storage Stability

Storage Stability Organism
-20 °C, lyophilized enzyme, more than 1 year without any loss in activity Aspergillus fumigatus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chitin + H2O swollen chitin, partially deacetylated chitin and lower activity towards colloidal chitin Aspergillus fumigatus ?
-
 ?
chitin + H2O swollen chitin, partially deacetylated chitin and lower activity towards colloidal chitin Aspergillus fumigatus YJ-407 ?
-
 ?
chitosan + H2O
-
 Aspergillus fumigatus ?
-
 ?
chitosan + H2O
-
 Aspergillus fumigatus YJ-407 ?
-
 ?
glycol chitin + H2O
-
 Aspergillus fumigatus ?
-
 ?
glycol chitin + H2O
-
 Aspergillus fumigatus YJ-407 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 46000, SDS-PAGE Aspergillus fumigatus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Aspergillus fumigatus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 40% of activity after 30 min retained Aspergillus fumigatus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Aspergillus fumigatus

pH Range

pH Minimum pH Maximum Comment Organism
4 8
-
 Aspergillus fumigatus

pI Value

Organism Comment pI Value Maximum pI Value
Aspergillus fumigatus isoelectric focusing
-
 6