Literature summary for 3.2.1.14 extracted from

Kirsch, C.; Hahlbrock, K.; Kombrink, E.
Purification and characterization of extracellular, acidic chitinase isoenzymes from elicitor-stimulated parsley cells (1993), Eur. J. Biochem., 213, 419-425.

Search for more literature for 3.2.1.14

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
extracellular	-	Petroselinum crispum	-	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
30400	-	x * 35100, isoenzyme C2, SDS-PAGE, x * 36800, isoenzyme C4, SDS-PAGE, x * 37000, isoenzyme C1, SDS-PAGE, x * 38200, isoenzyme C3, SDS-PAGE, x * 30400, isoenzyme A, SDS-PAGE, x * 32700, isoenzyme B, SDS-PAGE	Petroselinum crispum
32700	-	x * 35100, isoenzyme C2, SDS-PAGE, x * 36800, isoenzyme C4, SDS-PAGE, x * 37000, isoenzyme C1, SDS-PAGE, x * 38200, isoenzyme C3, SDS-PAGE, x * 30400, isoenzyme A, SDS-PAGE, x * 32700, isoenzyme B, SDS-PAGE	Petroselinum crispum
35100	-	x * 35100, isoenzyme C2, SDS-PAGE, x * 36800, isoenzyme C4, SDS-PAGE, x * 37000, isoenzyme C1, SDS-PAGE, x * 38200, isoenzyme C3, SDS-PAGE, x * 30400, isoenzyme A, SDS-PAGE, x * 32700, isoenzyme B, SDS-PAGE	Petroselinum crispum
36800	-	x * 35100, isoenzyme C2, SDS-PAGE, x * 36800, isoenzyme C4, SDS-PAGE, x * 37000, isoenzyme C1, SDS-PAGE, x * 38200, isoenzyme C3, SDS-PAGE, x * 30400, isoenzyme A, SDS-PAGE, x * 32700, isoenzyme B, SDS-PAGE	Petroselinum crispum
37000	-	x * 35100, isoenzyme C2, SDS-PAGE, x * 36800, isoenzyme C4, SDS-PAGE, x * 37000, isoenzyme C1, SDS-PAGE, x * 38200, isoenzyme C3, SDS-PAGE, x * 30400, isoenzyme A, SDS-PAGE, x * 32700, isoenzyme B, SDS-PAGE	Petroselinum crispum
38200	-	x * 35100, isoenzyme C2, SDS-PAGE, x * 36800, isoenzyme C4, SDS-PAGE, x * 37000, isoenzyme C1, SDS-PAGE, x * 38200, isoenzyme C3, SDS-PAGE, x * 30400, isoenzyme A, SDS-PAGE, x * 32700, isoenzyme B, SDS-PAGE	Petroselinum crispum

Organism

Organism	UniProt	Comment	Textmining
Petroselinum crispum	-	parsley, treated with fungal elicitor	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
glycoprotein	isoenzymes C1, C2, C4, isoenzymes C3 and C4 contain xylose complexes	Petroselinum crispum

Purification (Commentary)

Purification (Comment)	Organism
all six isoenzymes	Petroselinum crispum

Source Tissue

Source Tissue	Comment	Organism	Textmining
culture filtrate	-	Petroselinum crispum	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.0047	0.01	isoenzymes C2 and B, other isoenzymes in between	Petroselinum crispum

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
chitin + H2O	[acetyl-3H]chitin	Petroselinum crispum	N,N-diacetylchitobiose + N-acetylglucosamine	isoenzymes A and B chitotetraose and chitotriose main products, also chitobiose and higher chitooligomers, isoenzymes C1-C4 main product chitobiose, also N-acetylglucosamine and some chitotriose	?

Subunits

Subunits	Comment	Organism
?	x * 35100, isoenzyme C2, SDS-PAGE, x * 36800, isoenzyme C4, SDS-PAGE, x * 37000, isoenzyme C1, SDS-PAGE, x * 38200, isoenzyme C3, SDS-PAGE, x * 30400, isoenzyme A, SDS-PAGE, x * 32700, isoenzyme B, SDS-PAGE	Petroselinum crispum

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
4	-	isoenzyme A	Petroselinum crispum
5	-	isoenzyme B	Petroselinum crispum
6	-	isoenzyme C2	Petroselinum crispum
6.5	-	isoenzymes C1, C3	Petroselinum crispum
7	-	isoenzyme C4	Petroselinum crispum

pH Range

pH Minimum	pH Maximum	Comment	Organism
9	-	50% of activity isoenzymes C1-C4	Petroselinum crispum

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Release 2023.1 (January 2023)