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Literature summary for 3.2.1.14 extracted from

  • Cabib, E.
    Endochitinase from wheat germ (1988), Methods Enzymol., 161, 498-501.
No PubMed abstract available
Search for more literature for 3.2.1.14

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2
-
 chitin chitin concentration calculated as N-acetylglucosamine Triticum aestivum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29000
-
 gel filtration Triticum aestivum
30000
-
 SDS-PAGE Triticum aestivum
33000
-
 sedimentation equilibrium Triticum aestivum

Organism

Organism UniProt Comment Textmining
Triticum aestivum
-
 wheat
-

Purification (Commentary)

Purification (Comment) Organism
-
 Triticum aestivum

Source Tissue

Source Tissue Comment Organism Textmining
germ
-
 Triticum aestivum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
14.6
-
-
 Triticum aestivum

Storage Stability

Storage Stability Organism
-20°C or 2°C, several weeks, no loss of activity Triticum aestivum
-80°C, 7 years, no loss of activity Triticum aestivum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chitin + H2O
-
 Triticum aestivum N,N-diacetylchitobiose + N-acetylglucosamine
-
 ?
chitosan + H2O reacetylated Triticum aestivum chitosan oligosaccharides from diacetylchitobiose to tetraacetylchitotetraose ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Triticum aestivum

pH Range

pH Minimum pH Maximum Comment Organism
3 9
-
 Triticum aestivum