Any feedback?
Literature summary for 3.2.1.139 extracted from
- Expression and characterization of glycosyl hydrolase family 115 alpha-glucuronidase from Scheffersomyces stipitis (2016), Ind. Biotechnol., 12, 98-104 .
No PubMed abstract available
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Pichia pastoris strain X33 | Scheffersomyces stipitis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 8.2 | - |
aldotetraouronic acid | at pH 4.0 and 50°C | Scheffersomyces stipitis |  |
| 9 | - |
aldopentaouronic acid | at pH 4.0 and 50°C | Scheffersomyces stipitis | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Scheffersomyces stipitis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-Sepharose column chromatography | Scheffersomyces stipitis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| aldopentaouronic acid + H2O | high activity | Scheffersomyces stipitis | 4-O-methylglucuronic acid + ? | - |
? | |
| aldotetraouronic acid + H2O | highest activity | Scheffersomyces stipitis | 4-O-methylglucuronic acid + ? | - |
? | |
| beechwood xylan + H2O | low activity | Scheffersomyces stipitis | 4-O-methylglucuronic acid + ? | - |
? | |
| birchwood xylan + H2O | very low activity | Scheffersomyces stipitis | 4-O-methylglucuronic acid + ? | - |
? | |
| oat spelt xylan + H2O | negligible activity | Scheffersomyces stipitis | 4-O-methylglucuronic acid + ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 150000, SDS-PAGE | Scheffersomyces stipitis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Agu115 | - |
Scheffersomyces stipitis |
| glycosyl hydrolase family 115 alpha-glucuronidase | - |
Scheffersomyces stipitis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Scheffersomyces stipitis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 19.4 | - |
birchwood xylan | at pH 4.0 and 50°C | Scheffersomyces stipitis | |
| 24.2 | - |
beechwood xylan | at pH 4.0 and 50°C | Scheffersomyces stipitis | |
| 51.1 | - |
aldopentaouronic acid | at pH 4.0 and 50°C | Scheffersomyces stipitis | |
| 69.2 | - |
aldotetraouronic acid | at pH 4.0 and 50°C | Scheffersomyces stipitis | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4 | - |
- |
Scheffersomyces stipitis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 8.5 | - |
Scheffersomyces stipitis |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
