Any feedback?
Literature summary for 3.2.1.139 extracted from
- Characterization of a recombinant alpha-glucuronidase from Aspergillus fumigatus (2013), Fungal Biol., 117, 380-387.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| from strain A1163, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of His-tagged enzyme in Pichia pastoris | Aspergillus fumigatus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Aspergillus fumigatus | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 91730 | - |
recombinant mature enzyme, sequence calculation | Aspergillus fumigatus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus fumigatus | - |
- |
- |
| Aspergillus fumigatus A1163 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from Pichia pastoris by nickel affinity chromatography | Aspergillus fumigatus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| additional information | growth substrate is birchwood xylan | Aspergillus fumigatus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.29 | - |
recombinant His-tagged enzyme from Pichia pastoris, pH 5.0, 28°C | Aspergillus fumigatus |
| 1.09 | - |
fungus native culture supernatant, pH 5.0, 28°C | Aspergillus fumigatus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | alpha-glucuronidases act on xylan side-chains and release methyl glucuronic acid residues. The enzyme is active on xylooligosaccharides generated from birchwood glucuronoxylan by a family 10 endoxylanase and on a mixture of aldouronic acids. No activity with birchwood glucuronoxylan | Aspergillus fumigatus | ? | - |
? |  |
| additional information | alpha-glucuronidases act on xylan side-chains and release methyl glucuronic acid residues. The enzyme is active on xylooligosaccharides generated from birchwood glucuronoxylan by a family 10 endoxylanase and on a mixture of aldouronic acids. No activity with birchwood glucuronoxylan | Aspergillus fumigatus A1163 | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AguA | - |
Aspergillus fumigatus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Aspergillus fumigatus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 5 | - |
Aspergillus fumigatus |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | the enzyme is classified in family 67 of the glycosyl hydrolases | Aspergillus fumigatus |
| metabolism | the enzyme acts in synergy with endoxylanases and other xylanolytic enzymes in the biodegradation of xylan | Aspergillus fumigatus |
| additional information | the enzyme binds strongly to cellulose, thus it likely possesses a carbohydrate binding module | Aspergillus fumigatus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
