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Literature summary for 3.2.1.139 extracted from

  • Lee, C.C.; Wagschal, K.; Kibblewhite-Accinelli, R.E.; Orts, W.J.; Robertson, G.H.; Wong, D.W.
    An alpha-glucuronidase enzyme activity assay adaptable for solid phase screening (2009), Appl. Biochem. Biotechnol., 155, 314-320.
    View publication on PubMed
Search for more literature for 3.2.1.139

Application

Application Comment Organism
molecular biology screening instrument to find alpha-glucuronidase genes in DNA libraries in solid phase that enables higher throughput compared to liquid phase assays - screening of 50000 clones per 15-cm petri dish, addition of beta-xylosidase increases signal, the enzyme is important in facilitating the cellulose breakdown for biofuel production Geobacillus stearothermophilus

Cloned(Commentary)

Cloned (Comment) Organism
Escherichia coli BL21(DE3)pLysE, Luria-Bertani broth, 37°C, plasmid pET29-Gste-AG-his-S Geobacillus stearothermophilus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1,2-linked glucuronic acid of non-reducing xylose-oligosaccahrides + H2O Geobacillus stearothermophilus hemicellulose consists mostly of xylan, a polymer of beta-1,4-linked xylose residues, and has among others side chains of glucuronic acid, attached by 1,2-glycosidic bonds D-glucuronic acid + ?
-
 ?
1,2-linked glucuronic acid of non-reducing xylose-oligosaccahrides + H2O Geobacillus stearothermophilus T6 hemicellulose consists mostly of xylan, a polymer of beta-1,4-linked xylose residues, and has among others side chains of glucuronic acid, attached by 1,2-glycosidic bonds D-glucuronic acid + ?
-
 ?

Organism

Organism UniProt Comment Textmining
Geobacillus stearothermophilus Q09LY5 T6
-
Geobacillus stearothermophilus T6 Q09LY5 T6
-

Purification (Commentary)

Purification (Comment) Organism
soluble phase onto HisTrap HP column, eluted with imidazole gradient in sodium phosphate buffer, pH 8, and 300 mM sodium chloride Geobacillus stearothermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,2-linked glucuronic acid of non-reducing xylose-oligosaccahrides + H2O hemicellulose consists mostly of xylan, a polymer of beta-1,4-linked xylose residues, and has among others side chains of glucuronic acid, attached by 1,2-glycosidic bonds Geobacillus stearothermophilus D-glucuronic acid + ?
-
 ?
1,2-linked glucuronic acid of non-reducing xylose-oligosaccahrides + H2O hemicellulose consists mostly of xylan, a polymer of beta-1,4-linked xylose residues, and has among others side chains of glucuronic acid, attached by 1,2-glycosidic bonds Geobacillus stearothermophilus T6 D-glucuronic acid + ?
-
 ?
aldouronic acid + H2O mixtures of aldobiouronic, aldottriouronic, aldotetraouronic, and aldopentaouronic acid, glucuronic acid groups attached via 1,2-linkage to xylose or non-reducing terminal residue of xylose-oligosaccharides Geobacillus stearothermophilus D-xylose + 4-O-methyl-D-glucuronic acid direct product of aldobiouronic acid ?
aldouronic acid + H2O mixtures of aldobiouronic, aldottriouronic, aldotetraouronic, and aldopentaouronic acid, glucuronic acid groups attached via 1,2-linkage to xylose or non-reducing terminal residue of xylose-oligosaccharides Geobacillus stearothermophilus T6 D-xylose + 4-O-methyl-D-glucuronic acid direct product of aldobiouronic acid ?

Synonyms

Synonyms Comment Organism
alpha-glucuronidase
-
 Geobacillus stearothermophilus