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Literature summary for 3.2.1.139 extracted from

  • Choi, I.D.; Kim, H.Y.; Choi, Y.J.
    Gene cloning and characterization of alpha-glucuronidase of Bacillus stearothermophilus No. 236 (2000), Biosci. Biotechnol. Biochem., 64, 2530-2537.
    View publication on PubMed
Search for more literature for 3.2.1.139

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Geobacillus stearothermophilus

Inhibitors

Inhibitors Comment Organism Structure
Ag+ 1 mM, 58% inhibition Geobacillus stearothermophilus
Cu2+ 1 mM, complete inhibition Geobacillus stearothermophilus
Hg2+ 1 mM, complete inhibition Geobacillus stearothermophilus
Mn2+ 1 mM, 78% inhibition Geobacillus stearothermophilus
Pb2+ 1 mM, 53% inhibition Geobacillus stearothermophilus
Zn2+ 1 mM, 43% inhibition Geobacillus stearothermophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.78
-
 2-O-alpha-(4-O-methyl-alpha-D-glucopyranosyluronic)-D-xylobiose 40°C, pH 6.5 Geobacillus stearothermophilus

Localization

Localization Comment Organism GeneOntology No. Textmining
intracellular
-
 Geobacillus stearothermophilus 5622
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
77000
-
 2 * 77000, SDS-PAGE Geobacillus stearothermophilus
78156
-
 2 * 78156, calculated from sequence Geobacillus stearothermophilus
161000
-
 gel filtration Geobacillus stearothermophilus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Geobacillus stearothermophilus the enzyme is involved in hydrolysis of xylan. Presence of endoxylanase is critical for efficient alpha-glucuronidase activity, and efficient alpha-glucuronidase activity is essential for the complete hydrolysis of intact xylan ?
-
 ?

Organism

Organism UniProt Comment Textmining
Geobacillus stearothermophilus
-
 No. 236
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
15.3
-
 hydrolysis of 2-O-alpha-(4-O-methyl-alpha-D-glucopyranosyluronic)-D-xylobiose Geobacillus stearothermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-O-alpha-(4-O-methyl-alpha-D-glucopyranosyluronic)-D-xylobiose + H2O i.e. aldotriouronic acid Geobacillus stearothermophilus 4-O-methylglucuronic acid + D-xylobiose
-
 ?
aldobiouronic acid + H2O
-
 Geobacillus stearothermophilus ?
-
 ?
aldotetraouronic acid + H2O
-
 Geobacillus stearothermophilus ?
-
 ?
additional information the enzyme is involved in hydrolysis of xylan. Presence of endoxylanase is critical for efficient alpha-glucuronidase activity, and efficient alpha-glucuronidase activity is essential for the complete hydrolysis of intact xylan Geobacillus stearothermophilus ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 77000, SDS-PAGE Geobacillus stearothermophilus
dimer 2 * 78156, calculated from sequence Geobacillus stearothermophilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Geobacillus stearothermophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 4 h, 20% loss of activity Geobacillus stearothermophilus
50
-
 half-life: 50 min Geobacillus stearothermophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
 Geobacillus stearothermophilus

pI Value

Organism Comment pI Value Maximum pI Value
Geobacillus stearothermophilus calculated from sequence
-
 5.34