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Literature summary for 3.2.1.139 extracted from

  • Khandke, K.M.; Vithayathil, P.J.; Murthy, S.K.
    Purification and characterization of an alpha-D-glucuronidase from a thermophilic fungus, Thermoascus aurantiacus (1989), Arch. Biochem. Biophys., 274, 511-517.
    View publication on PubMed
Search for more literature for 3.2.1.139

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.145
-
 4-O-Methyl-glucuronosyl-xylotriose
-
 Thermoascus aurantiacus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
107000
-
 1 * 107000, SDS-PAGE Thermoascus aurantiacus
118000
-
 gel filtration Thermoascus aurantiacus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Thermoascus aurantiacus the three enzymes, xylanase, alpha-D-glucuronidase, and beta-glucosidase, together are responsible for complete degradation of larchwood xylan to xylose and 4-O-methyl-alpha-D-glucuronic acid ?
-
 ?

Organism

Organism UniProt Comment Textmining
Thermoascus aurantiacus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Thermoascus aurantiacus

Source Tissue

Source Tissue Comment Organism Textmining
culture filtrate
-
 Thermoascus aurantiacus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.98
-
-
 Thermoascus aurantiacus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-O-methyl-alpha-D-glucuronosyl-xyloheptaose + H2O
-
 Thermoascus aurantiacus 4-O-methyl-alpha-D-glucuronate + xyloheptaose
-
 ?
4-O-methyl-glucuronosyl-xylobiose + H2O
-
 Thermoascus aurantiacus 4-O-methyl-glucuronate + xylobiose
-
 ?
4-O-methyl-glucuronosyl-xylohexaose + H2O
-
 Thermoascus aurantiacus 4-O-methyl-glucuronate + xylohexaose
-
 ?
4-O-methyl-glucuronosyl-xylopentaose + H2O
-
 Thermoascus aurantiacus 4-O-methyl-glucuronate + xylopentaose
-
 ?
4-O-methyl-glucuronosyl-xylose + H2O
-
 Thermoascus aurantiacus 4-O-methyl-glucuronate + xylose
-
 ?
4-O-methyl-glucuronosyl-xylotetraose + H2O
-
 Thermoascus aurantiacus 4-O-methyl-glucuronate + xylotetraose
-
 ?
4-O-methyl-glucuronosyl-xylotriose + H2O
-
 Thermoascus aurantiacus 4-O-methyl-glucuronate + xylotriose
-
 ?
additional information the three enzymes, xylanase, alpha-D-glucuronidase, and beta-glucosidase, together are responsible for complete degradation of larchwood xylan to xylose and 4-O-methyl-alpha-D-glucuronic acid Thermoascus aurantiacus ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 107000, SDS-PAGE Thermoascus aurantiacus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
-
 Thermoascus aurantiacus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 75 40°C: about 35% of maximal activity, 75°C: about 30% of maximal activity Thermoascus aurantiacus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
 1 mg/ml of substrate protects against heat inactivation Thermoascus aurantiacus
50
-
 in absence of substrate, 6 h, 15% loss of activity Thermoascus aurantiacus
60
-
 in absence of substrate, t1/2: 192 min Thermoascus aurantiacus
60
-
 in absence of substrate, 6 h, 58% loss of activity Thermoascus aurantiacus
70
-
 in absence of substrate, t1/2: 28 min Thermoascus aurantiacus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
-
 Thermoascus aurantiacus

pH Range

pH Minimum pH Maximum Comment Organism
3 5.8 about 45% of maximal activity at pH 3 and at pH 5.8 Thermoascus aurantiacus