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Literature summary for 3.2.1.139 extracted from

  • Kawabata, Y.; Ono, K.; Gama, Y.; Yoshida, S.; Kobayashi, H.; Kusakabe, I.
    Purification and characterization of alpha-glucuronidase from snail acetone powder (1995), Biosci. Biotechnol. Biochem., 59, 1086-1090.
No PubMed abstract available
Search for more literature for 3.2.1.139

Inhibitors

Inhibitors Comment Organism Structure
Monoiodoacetic acid
-
 Helix pomatia
N-bromosuccinimide
-
 Helix pomatia
PCMB
-
 Helix pomatia
SDS
-
 Helix pomatia

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.13
-
 p-nitrophenyl alpha-D-glucopyranosyluronic acid
-
 Helix pomatia

Metals/Ions

Metals/Ions Comment Organism Structure
BaCl2 slight activation Helix pomatia
CaCl2 slight activation Helix pomatia
FeCl3 slight activation Helix pomatia

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
97000
-
 x * 97000, SDS-PAGE Helix pomatia
180000
-
 gel filtration Helix pomatia

Organism

Organism UniProt Comment Textmining
Helix pomatia
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Helix pomatia

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Helix pomatia

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-O-alpha-D-glucuronosyl-D-xylose + H2O
-
 Helix pomatia D-glucuronate + D-xylose
-
 ?
3-O-alpha-D-glucuronosyl-D-xylose + H2O
-
 Helix pomatia D-glucuronate + D-xylose
-
 ?
4-O-alpha-D-glucuronosyl-D-xylose + H2O
-
 Helix pomatia D-glucuronate + D-xylose
-
 ?
benzyl 4-O-alpha-D-glucuronosyl-beta-D-glucoside + H2O
-
 Helix pomatia ?
-
 ?
O-alpha-glucuronosyl alpha-D-glucuronide + H2O
-
 Helix pomatia D-glucuronate
-
 ?
p-nitrophenyl alpha-D-glucuronide + H2O
-
 Helix pomatia p-nitrophenol + D-glucuronate
-
 ?

Subunits

Subunits Comment Organism
? x * 97000, SDS-PAGE Helix pomatia

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 60 about 40% of maximal activity at 30°C and at 60°C Helix pomatia

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 stable up to Helix pomatia

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3
-
-
 Helix pomatia

pH Range

pH Minimum pH Maximum Comment Organism
2 4 pH 2: about 30% of maximal activity, pH 4: about 70% of maximal activity Helix pomatia

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 7 30°C, 2 h, stable Helix pomatia