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Literature summary for 3.2.1.136 extracted from

  • Suchova, K.; Kozmon, S.; Puchart, V.; Malovikova, A.; Hoff, T.; Morkeberg Krogh, K.B.R.; Biely, P.
    Glucuronoxylan recognition by GH 30 xylanases A study with enzyme and substrate variants (2018), Arch. Biochem. Biophys., 643, 42-49 .
    View publication on PubMed
Search for more literature for 3.2.1.136

Protein Variants

Protein Variants Comment Organism
R293A the mutation reduces the catalytic efficiency of the enzyme on 4-O-methylglucuronoxylan 18times, causes 15times decrease in specific activity on MeGlcA3Xyl4, but it does not influence low activity on linear xylooligosaccharides Dickeya chrysanthemi

Organism

Organism UniProt Comment Textmining
Dickeya chrysanthemi
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-O-methyl-D-glucuronoxylan + H2O special substrate Dickeya chrysanthemi ?
-
 ?
4-O-methyl-D-glucuronoxylan methyl ester + H2O
-
 Dickeya chrysanthemi ?
-
 ?
4-O-methylglucoxylan + H2O
-
 Dickeya chrysanthemi ?
-
 ?

Synonyms

Synonyms Comment Organism
glucuronoxylan xylanohydrolase
-
 Dickeya chrysanthemi
Xyn30A
-
 Dickeya chrysanthemi
XynA
-
 Dickeya chrysanthemi

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.15
-
 4-O-methylglucoxylan wild type enzyme, at pH 5.5 and 35°C Dickeya chrysanthemi
0.27
-
 4-O-methyl-D-glucuronoxylan methyl ester wild type enzyme, at pH 5.5 and 35°C Dickeya chrysanthemi
10.6
-
 4-O-methylglucoxylan mutant enzyme R293A, at pH 5.5 and 35°C Dickeya chrysanthemi
12.8
-
 4-O-methyl-D-glucuronoxylan methyl ester mutant enzyme R293A, at pH 5.5 and 35°C Dickeya chrysanthemi
22.9
-
 4-O-methyl-D-glucuronoxylan mutant enzyme R293A, at pH 5.5 and 35°C Dickeya chrysanthemi
34.1
-
 4-O-methyl-D-glucuronoxylan wild type enzyme, at pH 5.5 and 35°C Dickeya chrysanthemi