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Literature summary for 3.2.1.136 extracted from

  • Freire, F.; Verma, A.; Bule, P.; Alves, V.; Fontes, C.; Goyal, A.; Najmudin, S.
    Conservation in the mechanism of glucuronoxylan hydrolysis revealed by the structure of glucuronoxylan xylanohydrolase (CtXyn30A) from Clostridium thermocellum (2016), Acta Crystallogr. Sect. D, 72, 1162-1173.
    View publication on PubMed
Search for more literature for 3.2.1.136

Crystallization (Commentary)

Crystallization (Comment) Organism
modeling of structure. The protein displays a jelly roll beta-sandwich fold presenting two potential carbohydrate binding clefts, A and B. The cleft A, is located between two loops connecting b4-b5 and b8-b9 strands. Tyr28 and Phe84 present on these loops make a planar hydrophobic binding surface to accommodate sugar ring of ligand. The cleft B, is located on concave surface of beta-sandwich fold. Tyr34 and Tyr104 make a planar hydrophobic platform, which may be inaccessible to ligand due to hindrance by Pro68 Acetivibrio thermocellus

Protein Variants

Protein Variants Comment Organism
F84A mutant has completely lost its binding affinity towards both glucuronic acid as well as arabinose substituted xylans Acetivibrio thermocellus
Y28A mutant has completely lost its binding affinity towards both glucuronic acid as well as arabinose substituted xylans Acetivibrio thermocellus
Y28A/F28A mutant has completely lost its binding affinity towards both glucuronic acid as well as arabinose substituted xylans Acetivibrio thermocellus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 5 mM, slightly shifts melting temperature, does not affect structure stability and binding affinity significantly Acetivibrio thermocellus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
13800
-
-
 Acetivibrio thermocellus
14000
-
-
 Acetivibrio thermocellus

Organism

Organism UniProt Comment Textmining
Acetivibrio thermocellus A3DJS9
-
-
Acetivibrio thermocellus DSM 1237 A3DJS9
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-O-methylglucuronoxylan + H2O
-
 Acetivibrio thermocellus ?
-
 ?
beechwood xylan + H2O
-
 Acetivibrio thermocellus ?
-
 ?
beechwood xylan + H2O
-
 Acetivibrio thermocellus DSM 1237 ?
-
 ?
birchwood xylan + H2O
-
 Acetivibrio thermocellus ?
-
 ?
additional information isoform XynGH30 has high binding affinity with different xylans through an exothermic mechanism, with relatively higher affinity against arabinoxylans. The enzyme shows negligible affinity towards celluloses, pectins and other polysaccharides but predominantly interacts with ligands containing a xylan backbone (beta-1,4-xylopyranose) Acetivibrio thermocellus ?
-
 ?
additional information isoform XynGH30 has high binding affinity with different xylans through an exothermic mechanism, with relatively higher affinity against arabinoxylans. The enzyme shows negligible affinity towards celluloses, pectins and other polysaccharides but predominantly interacts with ligands containing a xylan backbone (beta-1,4-xylopyranose) Acetivibrio thermocellus DSM 1237 ?
-
 ?
oat spelt xylan + H2O
-
 Acetivibrio thermocellus ?
-
 ?
oat spelt xylan + H2O
-
 Acetivibrio thermocellus DSM 1237 ?
-
 ?
rye arabinoxylan + H2O
-
 Acetivibrio thermocellus ?
-
 ?
wheat arabinoxylan + H2O
-
 Acetivibrio thermocellus ?
-
 ?
wheat arabinoxylan + H2O
-
 Acetivibrio thermocellus DSM 1237 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 14000, SDS-PAGE, x * 13800, calculated including His-tag Acetivibrio thermocellus

Synonyms

Synonyms Comment Organism
XynGH30
-
 Acetivibrio thermocellus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
82
-
 melting temperature Acetivibrio thermocellus
85
-
 melting temperature, presence of 5 mM Ca2+ Acetivibrio thermocellus