Cloned (Comment) | Organism |
---|---|
gene ynfF, expression of His-tagged XynC in Escherichia coli strain BL21(DE3) | Bacillus subtilis |
Crystallization (Comment) | Organism |
---|---|
purified recombinant His-tagged XynC, 5 mg/ml protein in 20 mM HEPES, 150 mM NaCl pH 7.2, is mixed with 0.2 M sodium tartrate and 20% PEG 3350, which results in a crystal-positive condition with 0.1 M sodium malonate pH 7.0, X-ray diffraction structure determination and analysis at 1.64-2.7 A resolution, molecular replacement | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | Q45070 | subsp. subtilis, strain 168, gene ynfF | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged XynC from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Bacillus subtilis |
Synonyms | Comment | Organism |
---|---|---|
xylanase C | - |
Bacillus subtilis |
XynC | - |
Bacillus subtilis |