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Literature summary for 3.2.1.136 extracted from

  • Perez-Avalos, O.; Sanchez-Herrera, L.M.; Salgado, L.M.; Ponce-Noyola, T.
    A bifunctional endoglucanase/endoxylanase from Cellulomonas flavigena with potential use in industrial processes at different pH (2008), Curr. Microbiol., 57, 39-44.
    View publication on PubMed
Search for more literature for 3.2.1.136

Cloned(Commentary)

Cloned (Comment) Organism
Escherichia coli DH5alpha as transforming host, Escherichia coli XLI-Blue MRA (P2), cloning vector pBluescript SKII, 37 degrees Celsius, Luria-Bertoni broth Cellulomonas flavigena

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 cellulose 0.16 mg/ml, Vmax = 971 micromol/min/mg protein Cellulomonas flavigena
additional information
-
 xylose 0.72 mg/ml, Vmax = 2854 micromol/min/mg protein Cellulomonas flavigena

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
49000
-
 SDS-PAGE Cellulomonas flavigena

Organism

Organism UniProt Comment Textmining
Cellulomonas flavigena A1XM14 fragment of 387 bp, part of the gene cxo; CDBB-531
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Cellulomonas flavigena CDBB-531 A1XM14 fragment of 387 bp, part of the gene cxo; CDBB-531
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Purification (Commentary)

Purification (Comment) Organism
centrifuged supernatant concentrated with membrane filter, applied to Bio-Gel P100 gel filtration column, cellulolytic fractions pooled, lyophilized, dissolved, loaded onto hydroxylapatite column, last steps repeated, 4 degrees Celsius, purity monitored by SDS-PAGE and isoelectric focusing Cellulomonas flavigena

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Remazol brilliant blue-carboxymethylcellulose + H2O
-
 Cellulomonas flavigena glucose + cellobiose + cellotriose + cellotetraose + high-molecular-mass oligosaccharides cellulose activity ?
xylan + H2O xylan from birchwood Cellulomonas flavigena xylose + arabinose + xylobiose + high-molecular-mass oligosaccharides xylanase activity ?

Synonyms

Synonyms Comment Organism
beta-1,4-endoxylanase
-
 Cellulomonas flavigena
bifunctional cellulase/xylanase
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 Cellulomonas flavigena
endoglucanase
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 Cellulomonas flavigena
endoxylanase
-
 Cellulomonas flavigena

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
 for cellulase and xylanase activity Cellulomonas flavigena

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 70
-
 Cellulomonas flavigena

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 highest cellulase activity in citrate-phosphate buffer, in Tris-HCl buffer only 50% activity Cellulomonas flavigena
7
-
 highest cellulase activity in phosphate buffer, about 10% less activity than in citrate-phosphate buffer Cellulomonas flavigena
7
-
 highest xylanase activity in citrate-phosphate buffer, 10% less activity than in Tris-HCl Cellulomonas flavigena
9
-
 highest xylanase activity in Tris-HCl buffer Cellulomonas flavigena

pH Range

pH Minimum pH Maximum Comment Organism
5 10 pH 5-7: sodium citrate-phosphate buffer, pH 5-8: phosphate buffer, pH 7-10: Tris-HCl buffer Cellulomonas flavigena

pI Value

Organism Comment pI Value Maximum pI Value
Cellulomonas flavigena isoelectric focusing in 5% polyacrylamide gel, pH 3.5-10 Ampholine, 200 V, 50 min, bands visualized with Coomassie brilliant blue G250 or silver staining, standard proteins of pI 3.5-9.3
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 4.3