Literature summary for 3.2.1.136 extracted from

Dornez, E.; Cuyvers, S.; Gebruers, K.; Delcour, J.A.; Courtin, C.M.
Contribution of wheat endogenous and wheat kernel associated microbial endoxylanases to changes in the arabinoxylan population during breadmaking (2008), J. Agric. Food Chem., 56, 2246-2253.

Search for more literature for 3.2.1.136

Application

Application	Comment	Organism
food industry	endogenous endoxylanase activity during fermentation and storing can have negative effects on dough, the enzyme effect depends on the wheat variety's enzyme content and inhibitor content	Triticum aestivum

Inhibitors

Inhibitors	Comment	Organism	Structure
additional information	wheat kernels contain endogenous endoxylanase inhibitors that interfere with enzyme activity and differ between the wheat varieties	Triticum aestivum

Organism

Organism	UniProt	Comment	Textmining
Triticum aestivum	-	varieties Legat and Astuce, additionally microbial washed off activity of endoxylanase is investigated	-

Purification (Commentary)

Purification (Comment)	Organism
shaking of wheat kernels with acetate buffer (25 mM, pH 5.0, 0.02% sodium azide) for 17 h, liquid phase dialyzed against 10 mM sodium acetate buffer, pH 5.0 at 7 degrees Celsius for 24 h, dialysate concentrated by freeze-drying and resuspension in water	Triticum aestivum

Source Tissue

Source Tissue	Comment	Organism	Textmining
kernel	-	Triticum aestivum	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	only relative enzyme activities are measured: microbial endoxylanase activity is higher than endogenous enzyme activity, higher total endoxylanase activity in variety Legat than Astuce, significant lower enzyme activity in bread from washed kernels in Astuce variety not in Legat wheat	Triticum aestivum

Storage Stability

Storage Stability	Organism
Freeze drying and concentration of washing liquid from kernels does not change endoxylanase activity.	Triticum aestivum

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
arabinoxylan + H2O	azurine-cross-linked arabinoxylan	Triticum aestivum	reduced sugars	-	?

Synonyms

Synonyms	Comment	Organism
endoxylanase	-	Triticum aestivum

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Release 2023.1 (January 2023)