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Literature summary for 3.2.1.136 extracted from

  • Bennett, N.A.; Ryan, J.; Biely, P.; Vrsanska, M.; Kremnicky, L.; Macris, B.J.; Kekos, D.; Christakopoulos, P.; Katapodis, P.; Claeyssens, M.; Nerinckx, W.; Ntauma, P.; Bhat, M.K.
    Biochemical and catalytic properties of an endoxylanase purified from the culture filtrate of Thermomyces lanuginosus ATCC 46882 (1998), Carbohydr. Res., 306, 445-455.
    View publication on PubMed

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
26300
-
-
Thermomyces lanuginosus

Organism

Organism UniProt Comment Textmining
Thermomyces lanuginosus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Thermomyces lanuginosus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
888.8
-
-
Thermomyces lanuginosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme releases mainly xylose and xylobiose from beechwood 4-O-methyl-D-glucuronoxylan, O-acetyl-4-O-methyl-D-glucuronoxylan and rhodymenan, the enzyme releases an acidic xylooligosaccharide from 4-O-methyl-D-glucoronoxylan, and an isomeric xylotetraose and anisomeric xylopentaose from rhodymenan, enzyme hydrolyses [1-3H]-xylooligosaccharides Thermomyces lanuginosus additional information
-
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Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
75
-
-
Thermomyces lanuginosus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
5
-
at pH 10.0, enzyme is completely stable Thermomyces lanuginosus
60
-
between pH 5.0-9.0, enzyme is completely stable Thermomyces lanuginosus
85
-
between pH 5.5-9.0, enzyme shows about 50% loss of activity Thermomyces lanuginosus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 6.5
-
Thermomyces lanuginosus

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 9 60°C, enzyme is completely stable Thermomyces lanuginosus
5.5 9 85°C, enzyme shows about 50% of its original activity Thermomyces lanuginosus
10
-
55°C, enzyme is completely stable Thermomyces lanuginosus

pI Value

Organism Comment pI Value Maximum pI Value
Thermomyces lanuginosus
-
-
3.7