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Literature summary for 3.2.1.136 extracted from

  • Nishitani, K.; Nevins, D.J.
    Enzymic analysis of feruloylated arabinoxylans (Feraxan) derived from Zea mays cell walls. I. Purification of novel enzymes capable of dissociating Feraxan fragments from Zea mays coleoptile cell wall (1988), Plant Physiol., 87, 883-890.
    View publication on PubMedView publication on EuropePMC

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
x * 45000, SDS-PAGE Bacillus subtilis

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation industrial amylase preparation, Novo Ban L-120 Bacillus subtilis
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
feraxan + H2O i.e. feruloylated glucuronoarabinoxylans, highly specific endo-type hydrolase Bacillus subtilis ?
-
?
feraxan + H2O hydrolyzes feraxan both in intact maize coleophile cell wall and maize arabinoxylans extracted from cell walls with alkaline Bacillus subtilis ?
-
?
additional information not: cellulose, carboxymethyl cellulose, lichenan, oat or barley beta-glucan, laminarin, dextran, starch, citrus or maize pectin, polyuronides, arabinan, galactan, arabinogalactan, yeast mannan, galactomannan Bacillus subtilis additional information no products are mono-, di-, tri-, or tetramers of xylose and/or arabinose ?
additional information not: cellulose, carboxymethyl cellulose, lichenan, oat or barley beta-glucan, laminarin, dextran, starch, citrus or maize pectin, polyuronides, arabinan, galactan, arabinogalactan, yeast mannan, galactomannan Bacillus subtilis additional information products from hydrolysis of extracted maize arabinoxylan: major linkages in smaller fractions are terminal arabinofuranosyl, 5-linked arabinofuranosyl, 4-linked xylopyranoslyl, 3,4-linked xylopyranosyl and terminal glucuronosyl residues ?
additional information not: larch arabino-1,4-beta-D-xylan, Rhodymenia 1,3-1,4-beta-D-xylan Bacillus subtilis additional information no products are mono-, di-, tri-, or tetramers of xylose and/or arabinose ?
additional information not: larch arabino-1,4-beta-D-xylan, Rhodymenia 1,3-1,4-beta-D-xylan Bacillus subtilis additional information products from hydrolysis of extracted maize arabinoxylan: major linkages in smaller fractions are terminal arabinofuranosyl, 5-linked arabinofuranosyl, 4-linked xylopyranoslyl, 3,4-linked xylopyranosyl and terminal glucuronosyl residues ?

Subunits

Subunits Comment Organism
? x * 45000, SDS-PAGE Bacillus subtilis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Bacillus subtilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 7
-
Bacillus subtilis