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Literature summary for 3.2.1.135 extracted from
- Isolation and identification of a new Bacillus cereus strain and characterization of its neopullulanase (2015), Appl. Food Biotechnol., 2, 39-45 .
No PubMed abstract available
Application
| Application | Comment | Organism |
|---|---|---|
| biotechnology | thermostable debranching enzyme for biotechnology | Bacillus cereus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ammonium sulfate | 0.5 M, 40% inhibition | Bacillus cereus |  |
| Cu2+ | 0.2 mM, 86% inhibition | Bacillus cereus | |
| EDTA | 10 mM, 70% inhibition | Bacillus cereus | |
| Fe2+ | 0.2 mM, 21% inhibition | Bacillus cereus | |
| Mg2+ | 0.2 mM, 58% inhibition | Bacillus cereus | |
| Ni2+ | 0.2 mM, 29% inhibition | Bacillus cereus | |
| SDS | 1 mM, complete inhibition | Bacillus cereus | |
| Urea | 3 M, 60% inhibition | Bacillus cereus | |
| Zn2+ | 0.2 mM, 55% inhibition | Bacillus cereus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Bacillus cereus | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 2 mM, 1.6fold activation | Bacillus cereus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus cereus | - |
- |
- |
| Bacillus cereus SDK2 | - |
- |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 13.43 | - |
37°C, pH 7 | Bacillus cereus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pullulan + H2O | - |
Bacillus cereus | panose + ? | - |
? | |
| pullulan + H2O | - |
Bacillus cereus SDK2 | panose + ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Bacillus cereus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 70 | 20°C: about 45% of maximal activity, 70°C: about 65% of maximal activity | Bacillus cereus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Bacillus cereus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Bacillus cereus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9 | pH 6.0: about 60% of maximal activity, pH 9.0: about 50% of maximal activity | Bacillus cereus |
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