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Literature summary for 3.2.1.135 extracted from

  • Zareian, S.; Khajeh, K.; Ranjbar, B.; Dabirmanesh, B.; Ghollasi, M.; Mollania, N.
    Purification and characterization of a novel amylopullulanase that converts pullulan to glucose, maltose, and maltotriose and starch to glucose and maltose (2010), Enzyme Microb. Technol., 46, 57-63.
No PubMed abstract available
Search for more literature for 3.2.1.135

General Stability

General Stability Organism
2 mM EDTA and 2 mM phytic acid decreases the stability of the enzyme Geobacillus stearothermophilus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 5 mM Ca2+ increases the stability of the enzyme Geobacillus stearothermophilus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
100000
-
 1 * 100000, SDS-PAGE Geobacillus stearothermophilus

Organism

Organism UniProt Comment Textmining
Geobacillus stearothermophilus
-
-
-
Geobacillus stearothermophilus L14
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, DEAE-Sepharose column chromatography, and Sephadex G-100 gel filtration Geobacillus stearothermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
maltoheptaose + H2O
-
 Geobacillus stearothermophilus ?
-
 ?
maltoheptaose + H2O
-
 Geobacillus stearothermophilus L14 ?
-
 ?
maltohexaose + H2O
-
 Geobacillus stearothermophilus ?
-
 ?
maltohexaose + H2O
-
 Geobacillus stearothermophilus L14 ?
-
 ?
maltopentaose + H2O
-
 Geobacillus stearothermophilus ?
-
 ?
maltopentaose + H2O
-
 Geobacillus stearothermophilus L14 ?
-
 ?
maltotetraose + H2O
-
 Geobacillus stearothermophilus ?
-
 ?
maltotetraose + H2O
-
 Geobacillus stearothermophilus L14 ?
-
 ?
maltotriose + H2O
-
 Geobacillus stearothermophilus ?
-
 ?
additional information the enzyme is unable to degrade alpha-cyclodextrin and panose Geobacillus stearothermophilus ?
-
 ?
additional information the enzyme is unable to degrade alpha-cyclodextrin and panose Geobacillus stearothermophilus L14 ?
-
 ?
pullulan + H2O the enzyme attacks both alpha-D-(1->4) and alpha-D-(1->6) glycosidic linkages Geobacillus stearothermophilus D-glucose + maltose + maltotriose
-
 ?
soluble starch + H2O
-
 Geobacillus stearothermophilus D-glucose + maltose
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 100000, SDS-PAGE Geobacillus stearothermophilus

Synonyms

Synonyms Comment Organism
amylopullulanase
-
 Geobacillus stearothermophilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
-
 Geobacillus stearothermophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60 70 the irreversible thermoinactivation of the enzyme is recorded in 50 mM sodium acetate, pH 5.5 at 60, 70 and 80°C. Pullulanase retains 85% of its activity after 50 min of incubation at 60°C, its residual activities at 70 and 80°C after 50 min are 70 and 15%, respectively Geobacillus stearothermophilus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
62000
-
 pullulan
-
 Geobacillus stearothermophilus
111000
-
 soluble starch
-
 Geobacillus stearothermophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
 Geobacillus stearothermophilus

pH Range

pH Minimum pH Maximum Comment Organism
5 7
-
 Geobacillus stearothermophilus