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Literature summary for 3.2.1.135 extracted from
- Cooperativity and substrate specificity of an alkaline amylase and neopullulanase complex of Micrococcus halobius OR-1 (2001), Appl. Biochem. Biotechnol., 90, 233-249.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| formaldehyde | - |
Nesterenkonia halobia |  |
| additional information | not: 1-(dimethylamino-propyl)-3-ethyl carbodiimide-HCL | Nesterenkonia halobia |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 53000 | - |
SDS-PAGE | Nesterenkonia halobia |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pullulan + H2O | Nesterenkonia halobia | - |
panose + ? | - |
? | |
| pullulan + H2O | Nesterenkonia halobia OR-1 | - |
panose + ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Nesterenkonia halobia | - |
OR-1 | - |
| Nesterenkonia halobia OR-1 | - |
OR-1 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Nesterenkonia halobia |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Nesterenkonia halobia |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | enzyme hydrolyzes starch | Nesterenkonia halobia | ? | - |
? | |
| additional information | enzyme hydrolyzes not only alpha-1,4-glucosidic linkages but also specific alpha-1,6-glucosidic linkages of several branched oligosaccharides | Nesterenkonia halobia | ? | - |
? | |
| additional information | enzyme hydrolyzes starch | Nesterenkonia halobia OR-1 | ? | - |
? | |
| additional information | enzyme hydrolyzes not only alpha-1,4-glucosidic linkages but also specific alpha-1,6-glucosidic linkages of several branched oligosaccharides | Nesterenkonia halobia OR-1 | ? | - |
? | |
| pullulan + H2O | - |
Nesterenkonia halobia | panose + ? | - |
? | |
| pullulan + H2O | - |
Nesterenkonia halobia OR-1 | panose + ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Nesterenkonia halobia |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
half-life 36 h | Nesterenkonia halobia |
| 60 | - |
half-life 24 h | Nesterenkonia halobia |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Nesterenkonia halobia |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Nesterenkonia halobia |
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Release 2023.1 (January 2023)
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