Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Geobacillus stearothermophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
65000 | - |
enzyme likely to be present in monomer-dimer equilibrium with a molecular ratio of 1:9 in 50 mM sodium acetate buffer, pH 6.0, x * 65000, SDS-PAGE, x * 70156, MALDI-TOF MS analyzis | Geobacillus stearothermophilus |
70156 | - |
enzyme likely to be present in monomer-dimer equilibrium with a molecular ratio of 1:9 in 50 mM sodium acetate buffer, pH 6.0, x * 65000, SDS-PAGE, x * 70156, MALDI-TOF MS analyzis | Geobacillus stearothermophilus |
140000 | - |
gel filtration | Geobacillus stearothermophilus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
pullulan + H2O | Geobacillus stearothermophilus | - |
panose + ? | - |
? | |
pullulan + H2O | Geobacillus stearothermophilus IMA6503 | - |
panose + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | Q9AIV2 | IMA6503 | - |
Geobacillus stearothermophilus IMA6503 | Q9AIV2 | IMA6503 | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Geobacillus stearothermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acarbose + H2O | - |
Geobacillus stearothermophilus | pseudotrisaccharide + D-glucose | and transglycosylation of D-glucose to isoacarbose | ? | |
acarbose + H2O | - |
Geobacillus stearothermophilus IMA6503 | pseudotrisaccharide + D-glucose | and transglycosylation of D-glucose to isoacarbose | ? | |
beta-cyclomaltodextrin + H2O | - |
Geobacillus stearothermophilus | maltose + D-glucose | - |
? | |
beta-cyclomaltodextrin + H2O | - |
Geobacillus stearothermophilus IMA6503 | maltose + D-glucose | - |
? | |
additional information | the enzyme catalyses condensation between two maltose molecules and subsequent hydrolysis of the resulting 6-O-alpha-maltosylmaltose to D-glucose and panose, when maltose concentration is inceased to 20% | Geobacillus stearothermophilus | ? | - |
? | |
additional information | enzyme hydrolyzes starch | Geobacillus stearothermophilus | ? | - |
? | |
additional information | the enzyme catalyses condensation between two maltose molecules and subsequent hydrolysis of the resulting 6-O-alpha-maltosylmaltose to D-glucose and panose, when maltose concentration is inceased to 20% | Geobacillus stearothermophilus IMA6503 | ? | - |
? | |
additional information | enzyme hydrolyzes starch | Geobacillus stearothermophilus IMA6503 | ? | - |
? | |
pullulan + H2O | - |
Geobacillus stearothermophilus | panose + ? | - |
? | |
pullulan + H2O | - |
Geobacillus stearothermophilus IMA6503 | panose + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Geobacillus stearothermophilus |
monomer | - |
Geobacillus stearothermophilus |
More | enzyme likely to be present in monomer-dimer equilibrium with a molecular ratio of 1:9 in 50 mM sodium acetate buffer, pH 6.0, x * 65000, SDS-PAGE, x * 70156, MALDI-TOF MS analyzis | Geobacillus stearothermophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
- |
Geobacillus stearothermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Geobacillus stearothermophilus |