Cloned (Comment) | Organism |
---|---|
expression in Bacillus subtilis | Geobacillus stearothermophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
62000 | - |
SDS-PAGE | Geobacillus stearothermophilus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
pullulan + H2O | Geobacillus stearothermophilus | - |
panose + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Geobacillus stearothermophilus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Geobacillus stearothermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | enzyme hydrolyzes starch | Geobacillus stearothermophilus | ? | - |
? | |
pullulan + H2O | - |
Geobacillus stearothermophilus | panose + ? | - |
? | |
pullulan + H2O | - |
Geobacillus stearothermophilus | panose + ? | + maltose + D-glucose, in a molar ratio of 3:1:1, small amount | ? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Geobacillus stearothermophilus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 80 | 40°C: about 50% of activity maximum, 80°C: about 25% of activity maximum | Geobacillus stearothermophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
EDTA stabilizes against thermal inactivation | Geobacillus stearothermophilus |
60 | - |
60 min, about 10% loss of activity | Geobacillus stearothermophilus |
70 | - |
60 min, about 80% loss of activity | Geobacillus stearothermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Geobacillus stearothermophilus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 7 | pH 5: about 25% of activity maximum, pH 7: about 40% of activity maximum | Geobacillus stearothermophilus |