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Literature summary for 3.2.1.135 extracted from

  • Kuriki, T.; Okada, S.; Imanaka, T.
    New type of pullulanase from Bacillus stearothermophilus and molecular cloning and expression of the gene in Bacillus subtilis (1988), J. Bacteriol., 170, 1554-1559.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Bacillus subtilis Geobacillus stearothermophilus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
62000
-
SDS-PAGE Geobacillus stearothermophilus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
pullulan + H2O Geobacillus stearothermophilus
-
panose + ?
-
?

Organism

Organism UniProt Comment Textmining
Geobacillus stearothermophilus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Geobacillus stearothermophilus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Geobacillus stearothermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information enzyme hydrolyzes starch Geobacillus stearothermophilus ?
-
?
pullulan + H2O
-
Geobacillus stearothermophilus panose + ?
-
?
pullulan + H2O
-
Geobacillus stearothermophilus panose + ? + maltose + D-glucose, in a molar ratio of 3:1:1, small amount ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
Geobacillus stearothermophilus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 80 40°C: about 50% of activity maximum, 80°C: about 25% of activity maximum Geobacillus stearothermophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
EDTA stabilizes against thermal inactivation Geobacillus stearothermophilus
60
-
60 min, about 10% loss of activity Geobacillus stearothermophilus
70
-
60 min, about 80% loss of activity Geobacillus stearothermophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
Geobacillus stearothermophilus

pH Range

pH Minimum pH Maximum Comment Organism
5 7 pH 5: about 25% of activity maximum, pH 7: about 40% of activity maximum Geobacillus stearothermophilus