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Literature summary for 3.2.1.135 extracted from

  • Smith, K.A.; Salyers, A.A.
    Characterization of a neopullulanase and an alpha-glucosidase from Bacteroides thetaiotaomicron 95-1 (1991), J. Bacteriol., 173, 2962-2968.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.135

Application

Application Comment Organism
synthesis panose migth be used as an anticariogenic sweetener in foods, development of a system for continuous production of extremely high panose syrup from pullulan by employing the enzyme Bacteroides thetaiotaomicron

Protein Variants

Protein Variants Comment Organism
additional information diverse mutants are constructed and specific activities, sugar compositions of pullulan hydrolysate and hydrolysis activities toward alpha-(1-4) and alpha-(1-6)-glucosidic linkages analyzed Bacteroides thetaiotaomicron

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
70000
-
 gel filtration Bacteroides thetaiotaomicron

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
pullulan + H2O Bacteroides thetaiotaomicron
-
 panose + ?
-
 ?

Organism

Organism UniProt Comment Textmining
Bacteroides thetaiotaomicron
-
 95-1
-
Bacteroides thetaiotaomicron 95-1
-
 95-1
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bacteroides thetaiotaomicron

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 comparison of wild-type and mutant enzymes Bacteroides thetaiotaomicron

Storage Stability

Storage Stability Organism
-20°C, 20% glycerol, stable Bacteroides thetaiotaomicron

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
maltoheptaose + H2O
-
 Bacteroides thetaiotaomicron D-glucose + maltose + ?
-
 ?
maltohexaose + H2O
-
 Bacteroides thetaiotaomicron D-glucose + maltose + ?
-
 ?
maltopentaose + H2O
-
 Bacteroides thetaiotaomicron D-glucose + maltose + ?
-
 ?
maltotetraose + H2O
-
 Bacteroides thetaiotaomicron 2 D-glucose + maltose
-
 ?
maltotriose + H2O
-
 Bacteroides thetaiotaomicron maltose + D-glucose
-
 ?
additional information enzyme is able to cleave beta-cyclodextrin Bacteroides thetaiotaomicron ?
-
 ?
additional information enzyme also cleaves alpha-1,4-bonds in amylose and in oligosaccharides of maltotriose through maltoheptaose in chain length Bacteroides thetaiotaomicron ?
-
 ?
pullulan + H2O
-
 Bacteroides thetaiotaomicron panose + ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 70000, SDS-PAGE Bacteroides thetaiotaomicron