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Literature summary for 3.2.1.133 extracted from

  • Abdul Manas, N.H.; Pachelles, S.; Mahadi, N.M.; Illias, R.M.
    The characterisation of an alkali-stable maltogenic amylase from Bacillus lehensis G1 and improved malto-oligosaccharide production by hydrolysis suppression (2014), PLoS ONE, 9, e106481.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol about 120% activity at 1% (v/v) Alkalihalobacillus lehensis

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Alkalihalobacillus lehensis

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ about 76% residual activity at 5 mM Alkalihalobacillus lehensis
Co2+ about 13% residual activity at 5 mM Alkalihalobacillus lehensis
Cu2+ about 5% residual activity at 4 mM Alkalihalobacillus lehensis
Fe2+ about 36% residual activity at 5 mM Alkalihalobacillus lehensis
Li+ about 87% residual activity at 5 mM Alkalihalobacillus lehensis
Mg2+ about 84% residual activity at 5 mM Alkalihalobacillus lehensis
additional information not inhibited by EDTA and Tween 20 Alkalihalobacillus lehensis
Ni2+ about 2% residual activity at 5 mM Alkalihalobacillus lehensis
Pb2+ about 2% residual activity at 5 mM Alkalihalobacillus lehensis
phenylmethylsulfonyl fluoride about 2% residual activity at 1% (v/v) Alkalihalobacillus lehensis
SDS about 6% residual activity at 1% (v/v) Alkalihalobacillus lehensis
Zn2+ about 7% residual activity at 5 mM Alkalihalobacillus lehensis

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ about 162% activity at 5 mM Alkalihalobacillus lehensis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
68000
-
2 * 68000, SDS-PAGE Alkalihalobacillus lehensis
104700
-
calculated from gel filtration Alkalihalobacillus lehensis

Organism

Organism UniProt Comment Textmining
Alkalihalobacillus lehensis X5CPN2
-
-
Alkalihalobacillus lehensis G1 X5CPN2
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography and Superdex 200 gel filtration Alkalihalobacillus lehensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-cyclodextrin + H2O 80% activity compared to beta-cyclodextrin Alkalihalobacillus lehensis ?
-
?
amylopectin + H2O less than 2% activity compared to beta-cyclodextrin Alkalihalobacillus lehensis ?
-
?
amylopectin + H2O less than 2% activity compared to beta-cyclodextrin Alkalihalobacillus lehensis G1 ?
-
?
amylose + H2O 45% activity compared to beta-cyclodextrin Alkalihalobacillus lehensis ?
-
?
amylose + H2O 45% activity compared to beta-cyclodextrin Alkalihalobacillus lehensis G1 ?
-
?
beta-cyclodextrin + H2O 100% activity Alkalihalobacillus lehensis D-glucose + maltose + maltotriose + maltooligosaccharides
-
?
gamma-cyclodextrin + H2O 10% activity compared to beta-cyclodextrin Alkalihalobacillus lehensis ?
-
?
gamma-cyclodextrin + H2O 10% activity compared to beta-cyclodextrin Alkalihalobacillus lehensis G1 ?
-
?
maltotriose + H2O
-
Alkalihalobacillus lehensis maltose + D-glucose
-
?
maltotriose + H2O
-
Alkalihalobacillus lehensis G1 maltose + D-glucose
-
?
soluble starch + H2O 45% activity compared to beta-cyclodextrin Alkalihalobacillus lehensis ?
-
?
soluble starch + H2O 45% activity compared to beta-cyclodextrin Alkalihalobacillus lehensis G1 ?
-
?

Subunits

Subunits Comment Organism
homodimer 2 * 68000, SDS-PAGE Alkalihalobacillus lehensis

Synonyms

Synonyms Comment Organism
MAG1
-
Alkalihalobacillus lehensis
maltogenic amylase
-
Alkalihalobacillus lehensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
Alkalihalobacillus lehensis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
-20 40 the enzyme retains more than 50% of its activity at 40°C and 30°C for 10 min. The half-lives of the enzyme are 239.02 h, 126.03 h and 108.30 h at -20°C, 4°C and 20°C, respectively. However, the half-lives are dramatically reduced to 0.37 h and 0.04 h at 30°C and 40°C, respectively Alkalihalobacillus lehensis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
9.9
-
soluble starch at pH 7.0 and 40°C Alkalihalobacillus lehensis
12.5
-
beta-cyclodextrin at pH 7.0 and 40°C Alkalihalobacillus lehensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Alkalihalobacillus lehensis

pH Stability

pH Stability pH Stability Maximum Comment Organism
7 9 the enzyme is highly active and stable in a pH range from 7.0 to 9.0 Alkalihalobacillus lehensis