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Literature summary for 3.2.1.132 extracted from

  • Li, H.; Fei, Z.; Gong, J.; Yang, T.; Xu, Z.; Shi, J.
    Screening and characterization of a highly active chitosanase based on metagenomic technology (2015), J. Mol. Catal. B, 111, 29-35 .
No PubMed abstract available
Search for more literature for 3.2.1.132

Activating Compound

Activating Compound Comment Organism Structure
Triton X-100 1.0% v/v, 1.35fold increase in activity uncultured bacterium
Triton X-114 1.0% v/v, 1.38fold increase in activity uncultured bacterium
Tween 40 1.0% v/v, 1.85fold increase in activity uncultured bacterium
Tween 80 1.0% v/v, 1.29fold increase in activity uncultured bacterium

Inhibitors

Inhibitors Comment Organism Structure
Ag+ 5 mM, complete inhibition uncultured bacterium
Al3+ 5 mM, complete inhibition uncultured bacterium
Ba2+ 5 mM, 38% loss of activity uncultured bacterium
Co2+ 5 mM, 61% loss of activity uncultured bacterium
Cu2+ 5 mM, complete inhibition uncultured bacterium
EDTA 5 mM, 34% loss of activity uncultured bacterium
Fe2+ 5 mM, complete inhibition uncultured bacterium
Fe3+ 5 mM, complete inhibition uncultured bacterium
K+ 5 mM, 50% loss of activity uncultured bacterium
La3+ 5 mM, complete inhibition uncultured bacterium
Li+ 5 mM, 37% loss of activity uncultured bacterium
Mg2+ 5 mM, 46% loss of activity uncultured bacterium
Ni2+ 5 mM, complete inhibition uncultured bacterium
Pb2+ 5 mM, complete inhibition uncultured bacterium
PMSF 5 mM, 54% loss of activity uncultured bacterium
Rb+ 5 mM, 42% loss of activity uncultured bacterium
SDS 1.0% v/v, complete inhibition uncultured bacterium
Sn2+ 5 mM, 64% loss of activity uncultured bacterium
Zn2+ 5 mM, complete inhibition uncultured bacterium

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ 5 mM, activity is improved by 8.60% uncultured bacterium
Sr2+ 5 mM, activity is improved by 3.33% uncultured bacterium

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31000
-
 calculated from sequence, SDS-PAGE uncultured bacterium

Organism

Organism UniProt Comment Textmining
uncultured bacterium A0A0A7RCI7
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 uncultured bacterium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chitosan + H2O beta-D-GlcN-(1->4)-beta-D-GlcN-(1->4)-beta-D-GlcN, (GlcN)4 and (GlcN)5 are the major products uncultured bacterium beta-D-GlcN-(1->4)-beta-D-GlcN-(1->4)-beta-D-GlcN + beta-D-GlcN-(1->4)-beta-D-GlcN-(1->4)-beta-D-GlcN-(1->4)-beta-D-GlcN + beta-D-GlcN-(1->4)-beta-D-GlcN-(1->4)-beta-D-GlcN-(1->4)-beta-D-GlcN-(1->4)-beta-D-GlcN
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
 uncultured bacterium

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
35 60 35°C: about 30% of maximal activity, 60°C: about 50% of maximal activity uncultured bacterium

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 half-life: 20 h uncultured bacterium
55
-
 half-life: 20 h uncultured bacterium
60
-
 half-life: 30 min uncultured bacterium

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 uncultured bacterium

pH Range

pH Minimum pH Maximum Comment Organism
4.5 6.5 pH 4.5: about 50% of maximal activity, pH 6.5: about 60% of maximal activity uncultured bacterium