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Literature summary for 3.2.1.132 extracted from
- Single-step purification of chitosanases from Bacillus cereus using expanded bed chromatography (2016), Int. J. Biol. Macromol., 82, 291-298 .
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | 5 mM, about 40% loss of activity of chitosanase A, about 30% loss of activity of chitosanase B | Bacillus cereus |  |
| Fe2+ | 5 mM, about 30% loss of activity of chitosanase A, about 25% loss of activity of chitosanase B | Bacillus cereus | |
| Zn2+ | 5 mM, about 30% loss of activity of chitosanase A, about 40% loss of activity of chitosanase B | Bacillus cereus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | 5 mM, 1.6fold activation of chitosanase A, 1.2fold activation of chitosanase B | Bacillus cereus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 33000 | - |
chitosanase A | Bacillus cereus |
| 44000 | - |
chitosanase B | Bacillus cereus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus cereus | - |
- |
- |
| Bacillus cereus C-01 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bacillus cereus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| chitosan + H2O | - |
Bacillus cereus | ? | - |
? | |
| chitosan + H2O | - |
Bacillus cereus C-01 | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Chit A | - |
Bacillus cereus |
| Chit B | - |
Bacillus cereus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
chitosanase B | Bacillus cereus |
| 55 | - |
chitosanase A | Bacillus cereus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 70 | 30°C: about 45% of maximal activity, 70°C: about 40% of maximal activity, chitosanase B | Bacillus cereus |
| 40 | 70 | 40°C: about 65% of maximal activity, 70°C: about 45% of maximal activity, chitosanase A | Bacillus cereus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 60 | stable at, chitosanase A and chitosanase B | Bacillus cereus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
chitosanase A | Bacillus cereus |
| 6.5 | - |
chitosanase B | Bacillus cereus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 7.5 | chitosanase B | Bacillus cereus |
| 5.5 | 8 | chitosanase A | Bacillus cereus |
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Release 2023.1 (January 2023)
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