Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.132 extracted from

  • Qin, Z.; Chen, Q.; Lin, S.; Luo, S.; Qiu, Y.; Zhao, L.
    Expression and characterization of a novel cold-adapted chitosanase suitable for chitooligosaccharides controllable preparation (2018), Food Chem., 253, 139-147 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
heterologously expressed in Escherichia coli BL21 (DE3) Gynuella sunshinyii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
chitosan Km-value: 1.97 mg/ml, 30°C, pH 5.5 Gynuella sunshinyii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28000
-
SDS-PAGE Gynuella sunshinyii
29150
-
MALDI-TOF MS Gynuella sunshinyii
29700
-
calculated from sequence Gynuella sunshinyii

Organism

Organism UniProt Comment Textmining
Gynuella sunshinyii A0A0C5VFC0
-
-
Gynuella sunshinyii YC6258 A0A0C5VFC0
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Gynuella sunshinyii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chitosan + H2O the enzyme is an endo-type chitosanase, which cannot cleave the beta-1,4 linkage in (GlcN)2. The enzyme forms (GlcN)2 to (GlcN)7 as the main products after 30 min in the mild reaction condition. The hydrolytic products after 2 h incubation are mainly (GlcN)2, (GlcN)3, (GlcN)4 and a small quantity of (GlcN)5. There are only (GlcN)2 and (GlcN)3 detected after 6 h reaction Gynuella sunshinyii chitobiose + chitotriose + chitotetraose + chitopentaose + chitohexaose + chitoheptaose
-
?
chitosan + H2O the enzyme is an endo-type chitosanase, which cannot cleave the beta-1,4 linkage in (GlcN)2. The enzyme forms (GlcN)2 to (GlcN)7 as the main products after 30 min in the mild reaction condition. The hydrolytic products after 2 h incubation are mainly (GlcN)2, (GlcN)3, (GlcN)4 and a small quantity of (GlcN)5. There are only (GlcN)2 and (GlcN)3 detected after 6 h reaction Gynuella sunshinyii YC6258 chitobiose + chitotriose + chitotetraose + chitopentaose + chitohexaose + chitoheptaose
-
?

Subunits

Subunits Comment Organism
monomer 1 * 28000, SDS-PAGE Gynuella sunshinyii

Synonyms

Synonyms Comment Organism
GsCsn46A
-
Gynuella sunshinyii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
Gynuella sunshinyii

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
15 40 15°C: about 70% of maximal activity, 45°C: about 50% of maximal activity Gynuella sunshinyii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
35
-
stable up to 35°C Gynuella sunshinyii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
Gynuella sunshinyii

pH Range

pH Minimum pH Maximum Comment Organism
4.5 7 pH 4.5: about 50% of maximal activity, pH 7.0: about 50% of maximal activity Gynuella sunshinyii

pI Value

Organism Comment pI Value Maximum pI Value
Gynuella sunshinyii calculated from sequence
-
4.84