Cloned (Comment) | Organism |
---|---|
heterologously expressed in Escherichia coli BL21 (DE3) | Gynuella sunshinyii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
chitosan | Km-value: 1.97 mg/ml, 30°C, pH 5.5 | Gynuella sunshinyii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28000 | - |
SDS-PAGE | Gynuella sunshinyii |
29150 | - |
MALDI-TOF MS | Gynuella sunshinyii |
29700 | - |
calculated from sequence | Gynuella sunshinyii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gynuella sunshinyii | A0A0C5VFC0 | - |
- |
Gynuella sunshinyii YC6258 | A0A0C5VFC0 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Gynuella sunshinyii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
chitosan + H2O | the enzyme is an endo-type chitosanase, which cannot cleave the beta-1,4 linkage in (GlcN)2. The enzyme forms (GlcN)2 to (GlcN)7 as the main products after 30 min in the mild reaction condition. The hydrolytic products after 2 h incubation are mainly (GlcN)2, (GlcN)3, (GlcN)4 and a small quantity of (GlcN)5. There are only (GlcN)2 and (GlcN)3 detected after 6 h reaction | Gynuella sunshinyii | chitobiose + chitotriose + chitotetraose + chitopentaose + chitohexaose + chitoheptaose | - |
? | |
chitosan + H2O | the enzyme is an endo-type chitosanase, which cannot cleave the beta-1,4 linkage in (GlcN)2. The enzyme forms (GlcN)2 to (GlcN)7 as the main products after 30 min in the mild reaction condition. The hydrolytic products after 2 h incubation are mainly (GlcN)2, (GlcN)3, (GlcN)4 and a small quantity of (GlcN)5. There are only (GlcN)2 and (GlcN)3 detected after 6 h reaction | Gynuella sunshinyii YC6258 | chitobiose + chitotriose + chitotetraose + chitopentaose + chitohexaose + chitoheptaose | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 28000, SDS-PAGE | Gynuella sunshinyii |
Synonyms | Comment | Organism |
---|---|---|
GsCsn46A | - |
Gynuella sunshinyii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Gynuella sunshinyii |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
15 | 40 | 15°C: about 70% of maximal activity, 45°C: about 50% of maximal activity | Gynuella sunshinyii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
stable up to 35°C | Gynuella sunshinyii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
- |
Gynuella sunshinyii |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.5 | 7 | pH 4.5: about 50% of maximal activity, pH 7.0: about 50% of maximal activity | Gynuella sunshinyii |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Gynuella sunshinyii | calculated from sequence | - |
4.84 |