Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pseudomonas sp. A-01 |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion at 15°C | Pseudomonas sp. A-01 |
Protein Variants | Comment | Organism |
---|---|---|
D235A | with chitohexaose the mutant enzyme shows about 10% enhanced activity as compared to wild-type enzyme | Pseudomonas sp. A-01 |
D25A | with chitohexaose as substrate the mutant enzyme shows about 10% of the activity as compared to wild-type enzyme | Pseudomonas sp. A-01 |
D40A | with polymeric substrate the mutant enzyme shows less than 10% of the activity as compared to wild-type enzyme. With chitohexaose as substrate the mutant enzyme shows about 70% of the activity as compared to wild-type enzyme | Pseudomonas sp. A-01 |
D43A | with chitohexaose as substrate the mutant enzyme shows about 10% of the activity as compared to wild-type enzyme | Pseudomonas sp. A-01 |
D60E | with chitohexaose as substrate the mutant enzyme shows about 5% of the activity as compared to wild-type enzyme | Pseudomonas sp. A-01 |
D91A | with polymeric substrate the mutant enzyme shows about 10% enhanced activity as compared to wild-type enzyme. With chitohexaose as substrate the mutant enzyme shows about 70% of the activity as compared to wild-type enzyme | Pseudomonas sp. A-01 |
E120A | with polymeric substrate the mutant enzyme shows about 40% enhanced activity as compared to wild-type enzyme. With chitohexaose the mutant enzyme shows about 40% enhanced activity as compared to wild-type enzyme | Pseudomonas sp. A-01 |
E39A | with polymeric substrate the mutant enzyme shows about 50% enhanced activity as compared to wild-type enzyme | Pseudomonas sp. A-01 |
E63A | with polymeric substrate the mutant enzyme shows about 35% enhanced activity as compared to wild-type enzyme. With chitohexaose the mutant enzyme shows about 20% enhanced activity as compared to wild-type enzyme | Pseudomonas sp. A-01 |
H203A | with chitohexaose as substrate the mutant enzyme shows no activity | Pseudomonas sp. A-01 |
T58A | with chitohexaose as substrate the mutant enzyme shows about 90% of the activity as compared to wild-type enzyme | Pseudomonas sp. A-01 |
Y233A | with polymeric substrate the mutant enzyme shows less than 10% of the activity as compared to wild-type enzyme. With chitohexaose the mutant enzyme shows about 10% enhanced activity as compared to wild-type enzyme | Pseudomonas sp. A-01 |
Y37F | with chitohexaose as substrate the mutant enzyme shows about 95 % of the activity as compared to wild-type enzyme | Pseudomonas sp. A-01 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. A-01 | Q8KZM5 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
chitohexaose + H2O | - |
Pseudomonas sp. A-01 | ? | - |
? | |
chitosan + H2O | substrate recognition mechanismis for non-processive chitosanase is decribed | Pseudomonas sp. A-01 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
chitosanase OU01 | - |
Pseudomonas sp. A-01 |