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Literature summary for 3.2.1.132 extracted from

  • Zhou, Z.; Zhao, S.; Liu, Y.; Chang, Z.; Ma, Y.; Li, J.; Song, J.
    A highly conserved aspartic acid residue of the chitosanase from Bacillus sp. TS is involved in the substrate binding (2016), Appl. Biochem. Biotechnol., 180, 1167-1179 .
    View publication on PubMed
Search for more literature for 3.2.1.132

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21 (DE3) Bacillus sp. TS

Protein Variants

Protein Variants Comment Organism
D318A mutant enzyme retains 29.6% of wild-type activity Bacillus sp. TS
D318E mutant enzyme retains 16.4% of wild-type activity Bacillus sp. TS
D318K mutant enzyme retains 2.95% of wild-type activity Bacillus sp. TS
D318N mutant enzyme shows enhanced specific activity relative to wild-type chitosanase Bacillus sp. TS
D318R mutant enzyme retains 4.68% of wild-type activity Bacillus sp. TS

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 chitosan Km-value: 1.19 mg/ml, wild-type enzyme, pH 5.0, 50°C Bacillus sp. TS
additional information
-
 chitosan Km-value: 1.579 g/ml, mutant enzyme D318N, pH 5.0, 50°C Bacillus sp. TS
additional information
-
 chitosan Km-value: 12.33 mg/ml, mutant enzyme D318E, pH 5.0, 50°C Bacillus sp. TS
additional information
-
 chitosan Km-value: 12.67 mg/ml, mutant enzyme D318K, pH 5.0, 50°C Bacillus sp. TS
additional information
-
 chitosan Km-value: 18.5 mg/ml, mutant enzyme D318A, pH 5.0, 50°C Bacillus sp. TS
additional information
-
 chitosan Km-value: 5 mg/ml, mutant enzyme D318R, pH 5.0, 50°C Bacillus sp. TS

Organism

Organism UniProt Comment Textmining
Bacillus sp. TS A0A167KLN5
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bacillus sp. TS

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chitosan + H2O
-
 Bacillus sp. TS ?
-
 ?

Synonyms

Synonyms Comment Organism
CsnTS
-
 Bacillus sp. TS

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
57.5
-
 Tm-value of mutant enzymes D318R Bacillus sp. TS
57.7
-
 Tm-value of mutant enzymes D318K Bacillus sp. TS
63.6
-
 Tm-value of mutant enzyme D318A Bacillus sp. TS
67.1
-
 Tm-value of wild-type enzyme Bacillus sp. TS
67.5
-
 Tm-value of mutant enzyme D318N Bacillus sp. TS

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
19400
-
 chitosan pH 5.0, 50°C, mutant enzyme D318R Bacillus sp. TS
20500
-
 chitosan pH 5.0, 50°C, mutant enzyme D318E Bacillus sp. TS
21700
-
 chitosan pH 5.0, 50°C, mutant enzyme D318K Bacillus sp. TS
201000
-
 chitosan pH 5.0, 50°C, mutant enzyme D318A Bacillus sp. TS
505000
-
 chitosan pH 5.0, 50°C, wild-type enzyme Bacillus sp. TS
943000
-
 chitosan pH 5.0, 50°C, mutant enzyme D318N Bacillus sp. TS

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
 optimum pH for wild-type enzyme and mutant enzymes D318R and D318K Bacillus sp. TS

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
additional information
-
 chitosan Km-value: 10800 mg/s*ml, mutant enzyme D318A, pH 5.0, 50°C Bacillus sp. TS
additional information
-
 chitosan Km-value: 1630 mg/s*ml, mutant enzyme D318E, pH 5.0, 50°C Bacillus sp. TS
additional information
-
 chitosan Km-value: 1710 mg/s*ml, mutant enzyme D318K, pH 5.0, 50°C Bacillus sp. TS
additional information
-
 chitosan Km-value: 3880 mg/s*ml, mutant enzyme D318R, pH 5.0, 50°C Bacillus sp. TS
additional information
-
 chitosan Km-value: 424000 mg/s*ml, wild-type enzyme, pH 5.0, 50°C Bacillus sp. TS
additional information
-
 chitosan Km-value: 599000 mg/s*ml, mutant enzyme D318N, pH 5.0, 50°C Bacillus sp. TS