Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Streptomyces sp. |
Crystallization (Comment) | Organism |
---|---|
sitting drop vapor diffusion method, using 10% (w/v) MEPEG 5K, 300 mM tetramethylammonium chloride, and 100 mM Bis-Tris, pH 6.5, at 4°C | Streptomyces sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
chitosan + H2O | Streptomyces sp. | - |
? | - |
? | |
chitosan + H2O | Streptomyces sp. SirexAA-E | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces sp. | G2NRC4 | - |
- |
Streptomyces sp. SirexAA-E | G2NRC4 | - |
- |
Purification (Comment) | Organism |
---|---|
HisTrap column chromatography, and gel filtration | Streptomyces sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
chitosan + H2O | - |
Streptomyces sp. | ? | - |
? | |
chitosan + H2O | - |
Streptomyces sp. SirexAA-E | ? | - |
? | |
additional information | the enzyme does not react with chitin. Oligosaccharides with degree of polymerization above 4 are not hydrolyzed | Streptomyces sp. | ? | - |
? | |
additional information | the enzyme does not react with chitin. Oligosaccharides with degree of polymerization above 4 are not hydrolyzed | Streptomyces sp. SirexAA-E | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
SACTE_5457 | - |
Streptomyces sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6.5 | - |
chitosan | chitosan with 75-85% deacetylation, at pH 6.0 and 45°C | Streptomyces sp. | |
10 | - |
chitosan | chitosan with less than 75% deacetylation, at pH 6.0 and 45°C | Streptomyces sp. | |
11.2 | - |
chitosan | chitosan with more than 90% deacetylation, at pH 6.0 and 45°C | Streptomyces sp. |