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Literature summary for 3.2.1.132 extracted from

  • Lacombe-Harvey, M.E.; Fortin, M.; Ohnuma, T.; Fukamizo, T.; Letzel, T.; Brzezinski, R.
    A highly conserved arginine residue of the chitosanase from Streptomyces sp. N174 is involved both in catalysis and substrate binding (2013), BMC Biochem., 14, 23.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.132

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Streptomyces lividans strain TK24 and Escherichia coli Gold cells Streptomyces sp.

Protein Variants

Protein Variants Comment Organism
D40G the mutant shows drastic loss of activity Streptomyces sp.
D40G/R42E the mutant shows drastic loss of activity Streptomyces sp.
D40G/R42K the mutant shows drastic loss of activity Streptomyces sp.
R42E the mutant shows drastic loss of activity Streptomyces sp.
R42K the mutant shows drastic loss of activity Streptomyces sp.

Inhibitors

Inhibitors Comment Organism Structure
chitosan the wild type enzyme shows a Ki value of 1.547 mg/ml Streptomyces sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
chitosan + H2O Streptomyces sp. the wild type chitosanase shows a clear preference for chitosan with high degrees of N-deacetylation (84-97%) ?
-
 ?

Organism

Organism UniProt Comment Textmining
Streptomyces sp. P33665
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0012
-
 mutant enzyme D40G/R42E, at 37°C in 50 mM sodium acetate buffer (pH 5.5) Streptomyces sp.
0.018
-
 mutant enzyme D40G/R42K, at 37°C in 50 mM sodium acetate buffer (pH 5.5) Streptomyces sp.
0.9
-
 mutant enzyme R42E at 37°C in 50 mM sodium acetate buffer (pH 5.5) Streptomyces sp.
1.4
-
 mutant enzyme R42K, at 37°C in 50 mM sodium acetate buffer (pH 5.5) Streptomyces sp.
1.6
-
 mutant enzyme D40G, at 37°C in 50 mM sodium acetate buffer (pH 5.5) Streptomyces sp.
52.9
-
 wild type enzyme, at 37°C in 50 mM sodium acetate buffer (pH 5.5) Streptomyces sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chitosan + H2O the wild type chitosanase shows a clear preference for chitosan with high degrees of N-deacetylation (84-97%) Streptomyces sp. ?
-
 ?

Synonyms

Synonyms Comment Organism
Csn
-
 Streptomyces sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.9
-
 chitosan mutant enzyme R42K, at 37°C in 50 mM sodium acetate buffer (pH 5.5) Streptomyces sp.
1.6
-
 chitosan mutant enzyme R42E at 37°C in 50 mM sodium acetate buffer (pH 5.5) Streptomyces sp.
10.7
-
 chitosan wild type enzyme, at 37°C in 50 mM sodium acetate buffer (pH 5.5) Streptomyces sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
 Streptomyces sp.