KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.2 | - |
GlcN-beta-(1->4)-GlcN-beta-(1->4)-GlcN-beta-(1->4)-GlcN-beta-(1->4)-GlcN-beta-(1->4)-GlcN | at pH 5.7 and 37°C | Aspergillus fumigatus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
23525 | - |
x * 23525, MALDI-TOF mass spectrometry | Aspergillus fumigatus |
30000 | - |
x * 30000, SDS-PAGE | Aspergillus fumigatus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
chitosan + H2O | Aspergillus fumigatus | the enzyme hydrolyzes 70% deacetylated chitosan faster than fully deacetylated chitosan | ? | - |
? | |
chitosan + H2O | Aspergillus fumigatus ATCC 13073 | the enzyme hydrolyzes 70% deacetylated chitosan faster than fully deacetylated chitosan | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus fumigatus | - |
- |
- |
Aspergillus fumigatus ATCC 13073 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
CM Sephadex C50 gel filtration and Sephadex G-100 gel filtration | Aspergillus fumigatus |
Source Tissue | Comment | Organism | Textmining |
---|
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
7.59 | - |
culture filtrate, at pH 5.7 and 37°C | Aspergillus fumigatus |
8.8 | - |
after 1.16fold purification, at pH 5.7 and 37°C | Aspergillus fumigatus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
chitosan + H2O | the enzyme hydrolyzes 70% deacetylated chitosan faster than fully deacetylated chitosan | Aspergillus fumigatus | ? | - |
? | |
chitosan + H2O | the enzyme hydrolyzes 70% deacetylated chitosan faster than fully deacetylated chitosan | Aspergillus fumigatus ATCC 13073 | ? | - |
? | |
Glc-beta-(1->4)-Glc-beta-(1->4)-Glc-beta-(1->4)-GlcN-beta-(1->4) + H2O | - |
Aspergillus fumigatus | ? | - |
? | |
Glc-beta-(1->4)-Glc-beta-(1->4)-Glc-beta-(1->4)-GlcN-beta-(1->4) + H2O | - |
Aspergillus fumigatus ATCC 13073 | ? | - |
? | |
GlcN-beta-(1->4)-GlcN-beta-(1->4)-GlcN-beta-(1->4)-GlcN-beta-(1->4)-GlcN-beta-(1->4)-GlcN + H2O | best substrate | Aspergillus fumigatus | GlcN-beta-(1->4)-GlcN-beta-(1->4)-GlcN + GlcN-beta-(1->4)-GlcN + GlcN-beta-(1->4)-GlcN-beta-(1->4)-GlcN-beta-(1->4)-GlcN | GlcN-beta-(1->4)-Glc-beta-(1->4)-Glc-beta-(1->4) is the main product | ? | |
GlcN-beta-(1->4)-GlcN-beta-(1->4)-GlcN-beta-(1->4)-GlcN-beta-(1->4)-GlcN-beta-(1->4)-GlcNAc + H2O | - |
Aspergillus fumigatus | ? | - |
? | |
GlcN-beta-(1->4)-GlcN-beta-(1->4)-GlcNAc-beta-(1->4)-GlcN-beta-(1->4)-GlcN-beta-(1->4)-GlcN + H2O | - |
Aspergillus fumigatus | ? | - |
? | |
GlcN-beta-(1->4)-GlcN-beta-(1->4)-GlcNAc-beta-(1->4)-GlcN-beta-(1->4)-GlcN-beta-(1->4)-GlcN + H2O | - |
Aspergillus fumigatus ATCC 13073 | ? | - |
? | |
additional information | the enzyme does not hydrolyze colloidal chitin or carboxymethylcellulose | Aspergillus fumigatus | ? | - |
? | |
additional information | the enzyme splits GlcN-GlcN and GlcNAc-GlcN bonds but not GlcNAc-GlcNAc or GlcN-GlcNAc bonds | Aspergillus fumigatus | ? | - |
? | |
additional information | the enzyme does not hydrolyze colloidal chitin or carboxymethylcellulose | Aspergillus fumigatus ATCC 13073 | ? | - |
? | |
additional information | the enzyme splits GlcN-GlcN and GlcNAc-GlcN bonds but not GlcNAc-GlcNAc or GlcN-GlcNAc bonds | Aspergillus fumigatus ATCC 13073 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 30000, SDS-PAGE | Aspergillus fumigatus |
? | x * 23525, MALDI-TOF mass spectrometry | Aspergillus fumigatus |
Synonyms | Comment | Organism |
---|---|---|
chitosanase II | - |
Aspergillus fumigatus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Aspergillus fumigatus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 50 | about 45% activity at 30°C, about 85% activity at 35°C, 100% activity at 40°C, about 30% activity at 50°C | Aspergillus fumigatus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 80 | after 15 min incubation the enzyme shows 100% activity at 30°C, about 90% at 40°C, about 85% at 50°C, about 82% at 60°C, about 80% at 70°C, about 60% at 80°C, about 40° at 90°C, and about 20% activity at 100°C | Aspergillus fumigatus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Aspergillus fumigatus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
1 | 9 | the enzyme is stable and retains more than 80% of its original activity from pH 1.0-9.0 after 15 min | Aspergillus fumigatus |