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Literature summary for 3.2.1.132 extracted from
- Production, purification and characterization of a new chitosanase enzyme and improvement of chitosan pentamer and hexamer yield in an enzyme membrane reactor (2014), Biocatal. Biotransform., 32, 208-213.
No PubMed abstract available
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Streptomyces zaomyceticus | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 95000 | - |
gel filtration | Streptomyces zaomyceticus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| chitosan + H2O | Streptomyces zaomyceticus | - |
chitosan oligosaccharides | - |
? |  |
| chitosan + H2O | Streptomyces zaomyceticus C6 | - |
chitosan oligosaccharides | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces zaomyceticus | - |
- |
- |
| Streptomyces zaomyceticus C6 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| acetone fractionation and column chromatography | Streptomyces zaomyceticus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| chitosan + H2O | - |
Streptomyces zaomyceticus | chitosan oligosaccharides | - |
? | |
| chitosan + H2O | - |
Streptomyces zaomyceticus C6 | chitosan oligosaccharides | - |
? | |
| additional information | the enzyme is not able to degrade chitin | Streptomyces zaomyceticus | ? | - |
? | |
| additional information | the enzyme is not able to degrade chitin | Streptomyces zaomyceticus C6 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 95000, SDS-PAGE | Streptomyces zaomyceticus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
at pH 5.5 | Streptomyces zaomyceticus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
- |
90 | the enzyme is stable over a temperature range of 0-50°C and shows about 20% of the initial activity after heating at 90°C for 10 min | Streptomyces zaomyceticus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 6.5 | the enzyme is stable over a a pH range of 4.5-6.5 | Streptomyces zaomyceticus |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Streptomyces zaomyceticus | isoelectric focusing | - |
6.5 |
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Release 2023.1 (January 2023)
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